PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) Antibod
- 产品名称：
- PerCP/Cyanine5.5 anti-mouse CD326 (Ep-CAM) Antibod
- 产品类别：
- 抗体
- 产品编号：
- 118219
- 产品应用：
- 118219
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TE-71 thymic epithelial cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
- Application Notes
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of?frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
- Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
- Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
- Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
- Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
- Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
- Ishii K, et al. 2012. Development. 139:1734. PubMed
- Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
- Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
- Ding X, et al. 2015. Cancer Res. 75:330. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Brulois K, et al. 2020. Nat Commun. 3.095833333. PubMed
- Garo LP, et al. 2021. Nat Commun. 12:2419. PubMed
- Schneider C, et al. 2018. Cell. 174:271. PubMed
- Daskou M, et al. 2022. PLoS Pathog. 18:e1010160. PubMed
- Chen J, et al. 2021. Sci Adv. 7:. PubMed
- Gui J, et al. 2011. Int Immunol. 1.407638889. PubMed
- Kuwahara A, et al. 2020. Elife. 9:00. PubMed
- Mizraji* G, et al. 2013. J Vis Exp. 77: 50388. PubMed
- Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
- Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
- Xueyang Yu et al. 2017. Immunity. 47(5):903-912 . PubMed
- Kurowska-Stolarska M, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31132-0. PubMed
- Nekritz EA, et al. 2021. EMBO Rep. 22:e53201. PubMed
- Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed
- Gross KM, et al. 2019. Cell Rep. 28:394. PubMed
- Yu S, et al. 2018. Cell Stem Cell. 23:46. PubMed
- Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
- RRID
- AB_2098647 (BioLegend Cat. No. 118219) AB_2246499 (BioLegend Cat. No. 118220)
- Structure
- 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
- Distribution
-
Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
- Function
- Mediates calcium-independent homophilic cell-cell adhesion.
- Interaction
- CD326 displays hemophilic binding.
- Ligand/Receptor
- CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.
- Gene ID
- 17075 View all products for this Gene ID
- UniProt
- View information about CD326 on UniProt.org
