PE anti-mouse CD326 (Ep-CAM) Antibody
- 产品名称：
- PE anti-mouse CD326 (Ep-CAM) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 118205
- 产品应用：
- 118205
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TE-71 thymic epithelial cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of?frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.
- Application References
(PubMed link indicates BioLegend citation) -
- Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
- Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
- Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
- Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
- Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
- Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
- Ishii K, et al. 2012. Development. 139:1734. PubMed
- Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
- Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
- Ding X, et al. 2015. Cancer Res. 75:330. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Kimura S, et al. 2020. Nat Commun. 0.620833333. PubMed
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- Yin N et al. 2019. Cancer Cell. 36(2):156-167 . PubMed
- Schwartz AJ, et al. 2021. Nat Metab. 3:969. PubMed
- Skibba ME, et al. 2021. Respir Res. 22:315. PubMed
- Yu D, et al. 2013. Mol Cell Biol. 33:1845. PubMed
- Kim S, et al. 2011. PLoS One. 6:e19310. PubMed
- Hao F, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
- Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
- Parker KA, et al. 2021. Mol Cancer Res. 19:2068. PubMed
- Bahar Halpern K, et al. 2020. Nat Commun. 11:1936. PubMed
- Airik M, et al. 2020. Hum Mol Genet. 29:3064. PubMed
- Reinfeld BI, et al. 2021. Nature. 593:282. PubMed
- Krueger A, et al. 2022. Front Microbiol. 12:789042. PubMed
- Scur M, et al. 2022. Nat Commun. 13:7272. PubMed
- Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
- Lee J, et al. 2013. Am J Respir Cell Mol Biol. 48:288. PubMed
- Tan C, et al. 2020. Front Cell Dev Biol. 8:579157. PubMed
- Yang Y, et al. 2021. Cell Reports. 34(10):108822. PubMed
- Tateishi R, et al. 2015. PLoS One. 10: e0141650. PubMed
- Mello AM, et al. 2022. Oncogenesis. 11:56. PubMed
- He B, et al. 2018. Nat Commun. 9:5240. PubMed
- Shigeyasu K, et al. 2015. Gut. 64:627. PubMed
- Zheng C, et al. 2022. Acta Pharm Sin B. 12:3726. PubMed
- Li Q, et al. 2022. J Biol Chem. :101753. PubMed
- Fujino N, et al. 2019. J Exp Med. 216:2184. PubMed
- Edelson B, et al. 2011. PLoS One. 6:e25660. PubMed
- Kudira R, et al. 2021. Bio Protoc. 11:e4192. PubMed
- Ichinose M, et al. 2021. Sci Rep. 7200:11. PubMed
- Avgustinova A, et al. 2021. Cell Stem Cell. . PubMed
- Pastor W, et al. 2014. Nat Commun. 12:5795. PubMed
- Filbey KJ, et al. 2019. Front Immunol. 10:2375. PubMed
- Barr J, et al. 2022. Elife. 11:. PubMed
- RRID
- AB_1134176 (BioLegend Cat. No. 118205) AB_1134172 (BioLegend Cat. No. 118206)
- Structure
- 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
- Distribution
-
Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
- Function
- Mediates calcium-independent homophilic cell-cell adhesion.
- Interaction
- CD326 displays hemophilic binding.
- Ligand/Receptor
- CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.
- Gene ID
- 17075 View all products for this Gene ID
- UniProt
- View information about CD326 on UniProt.org
