FITC anti-mouse CD326 (Ep-CAM) Antibody
- 产品名称：
- FITC anti-mouse CD326 (Ep-CAM) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 118207
- 产品应用：
- 118207
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TE-71 thymic epithelial cell line
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of?frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.
- Application References
(PubMed link indicates BioLegend citation) -
- Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
- Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
- Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
- Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
- Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
- Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
- Ishii K, et al. 2012. Development. 139:1734. PubMed
- Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
- Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
- Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
- Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
- Ding X, et al. 2015. Cancer Res. 75:330. PubMed
- Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
- Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
- Product Citations
-
- Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
- Poh AR, et al. 2017. Cancer Cell. 31:563. PubMed
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- Kurosawa M, et al. 2021. Int J Mol Sci. 22:. PubMed
- Saenz F, et al. 2014. PLoS One. 9:97666. PubMed
- Jard T, et al. 2020. Cell Stem Cell. 27(4):646-662.e7. PubMed
- Bittner-Eddy P, et al. 2016. J Immunol. 197: 1435 - 1446. PubMed
- Cleary A, et al. 2014. Nature. 508:113. PubMed
- Atapattu L, et al. 2016. J Exp Med. 213: 1741 - 1757. PubMed
- Helm M, et al. 2022. Life (Basel). 12:. PubMed
- Panciera T, et al. 2020. Nat Mater. 1.345138889. PubMed
- Wlodarska M et al. 2017. Cell host & microbe. 22(1):25-37 . PubMed
- Emg?rd J, et al. 2018. Immunity. 143:419. PubMed
- Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
- Furstenberg R, et al. 2011. Am J Physiol Gastrointest Liver Physiol. 300:G409. PubMed
- Choi J, et al. 2021. Nat Cell Biol. 23:953. PubMed
- Tomlinson KL, et al. 2021. Nat Commun. 12:1399. PubMed
- Yamamoto M, et al. 2019. Commun Biol. 2:292. PubMed
- Santos CP et al. 2019. Nat Commun. 10(1):4407 . PubMed
- Watson JK, et al. 2020. Sci Rep. 10:10490. PubMed
- Bauer T, et al. 2021. Life (Basel). 11:. PubMed
- Sun L, et al. 2015. Sci Rep. 5: 14871. PubMed
- Kim SM, et al. 2019. FASEB J. 33:13386. PubMed
- Pervolarakis N, et al. 2020. Cell Rep. 108273:33. PubMed
- Zhang Q, et al. 2021. Front Cell Dev Biol. 9:655552. PubMed
- Shibata T, et al. 2020. J Clin Invest. 130:3021. PubMed
- Kamata T, et al. 2020. Cell Reports. 31(12):107802. PubMed
- Fukushima K, et al. 2020. Immunity. 52(3):542-556. PubMed
- Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
- King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
- Matern M, et al. 2017. Sci Rep. 7:42079. PubMed
- Sato Y, et al. 2018. JCI Insight. 3:. PubMed
- DiMarco AV, et al. 2022. Cancer Immunol Res. 10:70. PubMed
- Zhao Q, et al. 2020. Cancer Res. 80:5531. PubMed
- Kim SH, et al. 2020. Neoplasia. 1.3375. PubMed
- Sfakianos JP, et al. 2020. Nat Commun. 2.222222222. PubMed
- Matei DE, et al. 2021. Med (N Y). 2:864. PubMed
- RRID
- AB_1134106 (BioLegend Cat. No. 118207) AB_1134107 (BioLegend Cat. No. 118208)
- Structure
- 40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
- Distribution
-
Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.
- Function
- Mediates calcium-independent homophilic cell-cell adhesion.
- Interaction
- CD326 displays hemophilic binding.
- Ligand/Receptor
- CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.
- Gene ID
- 17075 View all products for this Gene ID
- UniProt
- View information about CD326 on UniProt.org
