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Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) Ant
产品名称：
Alexa Fluor® 647 anti-mouse CD326 (Ep-CAM) Ant
产品类别：
抗体
产品编号：
118211
产品应用：
118211
[价格]
规格 价格 库存
25µg ￥ 1488 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD326 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC?- Verified

SB - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per 106 cells in 100 ?l volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 ?g/ml is suggested.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of?frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  14. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed
  2. Sandovici I, et al. 2022. Dev Cell. 57:63. PubMed
  3. Bilate AM, et al. 2020. Immunity. 53(5):1001-1014.e20. PubMed
  4. Davis F, et al. 2015. Proc Natl Acad Sci U S A. 112:5827. PubMed
  5. Molaro A, et al. 2014. Genes Dev. 28:1544. PubMed
  6. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  7. Strunz M, et al. 2020. Nat Commun. 2.929861111. PubMed
  8. McClendon J, et al. 2017. Am J Pathol. 10.1016/j.ajpath.2017.04.012. PubMed
  9. Blijswijk J, et al. 2015. J Immunol. 194:307. PubMed
  10. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  11. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  12. Riemondy KA, et al. 2019. JCI Insight. 5:. PubMed
  13. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  14. Burns KA, et al. 2018. Endocrinology. 159:103. PubMed
  15. Cheng Q, et al. 2020. Nat Nanotechnol. 0.842361111. PubMed
  16. Progatzky F, et al. 2021. Nature. 599:125. PubMed
  17. Cohen SB et al. 2018. Cell host & microbe. 24(3):439-446 . PubMed
  18. Campbell C et al. 2018. Immunity. 48(6):1245-1257 . PubMed
  19. Xiong H, et al. 2021. Theranostics. 11:1594. PubMed
  20. Zhang J, et al. 2018. Sci Rep. 8:2373. PubMed
  21. Dravis C et al. 2018. Cancer cell. 34(3):466-482 . PubMed
  22. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  23. Heijden M, et al. 2016. Nat Commun. 7:10916. PubMed
  24. Moon S, et al. 2021. J Exp Med. 218:. PubMed
  25. Hegab A, et al. 2015. Stem Cell Res. 15: 109-121. PubMed
  26. Shaffer M, et al. 2010. PLoS One. 5:e12404. PubMed
  27. Kolodkin-Gal D, et al. 2021. Gut. Online ahead of print. PubMed
  28. Ma Z, et al. 2022. Methods Mol Biol. 2471:49. PubMed
  29. Tang R, et al. 2020. Elife. 9:00. PubMed
  30. Giraddi R, et al. 2015. Nat Commun. 6: 8487. PubMed
  31. Bernstock JD, et al. 2019. J Allergy Clin Immunol. 145:358. PubMed
  32. Wasik K, et al. 2015. Genes Dev. 29: 1403 - 1415. PubMed
  33. Stewart AS, et al. 2021. Am J Physiol Gastrointest Liver Physiol. 321:G588. PubMed
  34. Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
  35. Miragaia RJ, et al. 2018. Sci Rep. 8:685. PubMed
  36. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  37. Fang Y, et al. 2022. Development. 149:. PubMed
  38. Liu Y, et al. 2020. Cell. 183(6):1665-1681.e18. PubMed
  39. Gaiser M, et al. 2012. Proc Natl Acad Sci U S A. 1.034027778. PubMed
  40. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  41. Bretz N, et al. 2014. Immunol Lett. 161:140. PubMed
  42. Giraddi RR et al. 2018. Cell reports. 24(6):1653-1666 . PubMed
  43. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  44. Oni TE, et al. 2020. J Exp Med. :217. PubMed
  45. Chung CY et al. 2019. Cell Rep. 29(2):495-510 . PubMed
RRID
AB_1134104 (BioLegend Cat. No. 118211) AB_1134101 (BioLegend Cat. No. 118212)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

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