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Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) Ant
产品名称：
Alexa Fluor® 488 anti-mouse CD326 (Ep-CAM) Ant
产品类别：
抗体
产品编号：
118210
产品应用：
118210
[价格]
规格 价格 库存
100µg ￥ 3180 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
IHC-F, 3D IHC?- Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume.?For immunohistochemical staining on frozen tissue sections, a concentration range of 2.5 - 10 ?g/ml is suggested.?For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of?frozen sections: acetone fixed1, with or without OCT embedding2,4, and spatial biology (IBEX)13,14.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
  13. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  14. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed
  2. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  3. Lyons J, et al. 2018. PLoS Biol. 16:e2002417. PubMed
  4. Baptista AP et al. 2019. Immunity. 50(5):1188-1201 . PubMed
  5. Weiner AI, et al. 2019. NPJ Regen Med. 4:17. PubMed
  6. Donati Y, et al. 2020. Am J Physiol Lung Cell Mol Physiol. L619:318. PubMed
  7. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  8. Sakamoto K, et al. 2021. Immunity. 54:2321. PubMed
  9. Sung PS, et al. 2022. JCI Insight. :. PubMed
  10. Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
  11. Coulombe P et al. 2019. Cell reports. 27(6):1769-1780 . PubMed
  12. Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
  13. Tomic G, et al. 2018. Cell Stem Cell. 1.261111111. PubMed
  14. Kojima T, et al. 2016. Sci Rep. 6:36457. PubMed
  15. Pinho AV, et al. 2018. Nat Commun. 9:5083. PubMed
  16. Ravindran A, et al. 2014. Carcinogenesis. 35:959. PubMed
RRID
AB_1134099 (BioLegend Cat. No. 118210)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

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