PE anti-rat CD8a Antibody -上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 1404	1

Product Details

Verified Reactivity

Rat

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

CD8⁺ Wistar rat splenic T cell hybridomas

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, blocking¹ of allogeneic responses, and immunohistochemical staining of acetone-fixed frozen sections. The LEAF? purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 200612).

Application References (PubMed link indicates BioLegend citation)

Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841.
Xie L, et al. 2014. J Immunol. 192:6009. PubMed

Product Citations

Xie L, et al. 2014. J Immunol. 192:6009. PubMed
Calik M, et al. 2015. ASN Neuro. 7: 1759091415595726. PubMed
Zhang HC, et al. 2018. Int J Mol Med. 41:13. PubMed

RRID

AB_313878 (BioLegend Cat. No. 200607) AB_313879 (BioLegend Cat. No. 200608)

Antigen Details

Structure: Ig superfamily, CD8α chain
Distribution: Most thymocytes, T cell subset; activated CD4⁺ T cells (not resting), most NK cells and intestinal intraepithelial cells express the CD8α chain without the CD8β chain
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Cell Type: Thymocytes, T cells, NK cells, Epithelial cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Dallman MJ, et al. 1982. Eur. J. Immunol. 12:511.
3. Cantrell DA, et al. 1982. Immunol. 45:97.
4. Lyscom N, et al. 1982. Immunol. 45:775.
5. Johnson P, et al. 1985. EMBO. J. 4:2539.
6. Barclay AN. 1981. Immunol. 42:593.
Gene ID: 24930 View all products for this Gene ID
UniProt: View information about CD8a on UniProt.org

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