Purified anti-human CD32 Antibody
- 产品名称：
- Purified anti-human CD32 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 303201
- 产品应用：
- 303201
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of acetone-fixed frozen tissue sections.?
Clone FUN-2 recognizes both CD32A and CD32B.
- Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. 1997. Leucocyte Typing VI Garland Press. London.
- Lerino F, et al. 1993. J. Immunol. 150:1794.
- Personal communication.
- van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol. 25:717.PubMed
- Smeltz RB. 2007. J. Immunol. 178:4786.
- Satta N, et al. 2011. Blood. 117:5223. PubMed.
- Product Citations
-
- Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed
- Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
- Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed
- Li L, et al. 2019. Cell Stem Cell. 24:271. PubMed
- van Tits L, et al. 2005. Arterioscler Thromb Vasc Biol . 25:717. PubMed
- Syrimi E, et al. 2021. iScience. 24:103215. PubMed
- Prodjinotho UF, et al. 2017. PLoS Negl Trop Dis. 11:e0005777. PubMed
- Satta N, et al. 2011. Blood. 117:5523. PubMed
- Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
- Combes AJ, et al. 2021. Nature. 591:124. PubMed
- Doni A, et al. 2021. Nat Commun. 12:3739. PubMed
- Chevrier S, et al. 2021. Cell Reports Medicine. 2(1):100166. PubMed
- Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
- Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
- RRID
- AB_314333 (BioLegend Cat. No. 303201) AB_314334 (BioLegend Cat. No. 303202)
- Structure
- Ig superfamily, polymorphic transmembrane glycoprotein, 40 kD
- Distribution
-
Monocytes, granulocytes, B cells, platelets
- Function
- Endocytosis, cytotoxicity, platelet aggregation, B cell function
- Ligand/Receptor
- Aggregated IgG, immune complex
- Cell Type
- B cells, Dendritic cells, Granulocytes, Monocytes, Platelets
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Fc Receptors
- Antigen References
-
1. Stuart S, et al. 1989. EMBO J. 8:3657.
2. Huang Y, et al. 1999. Scand. J. Immunol. 49:177.
3. Hisaka H, et al. 1999. Pathobiology 67:92.
- Gene ID
- 2212 View all products for this Gene ID
- UniProt
- View information about CD32 on UniProt.org
