Purified anti-human CD69 Antibody
- 产品名称：
- Purified anti-human CD69 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 310902
- 产品应用：
- 310902
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
CyTOF? - Verified
IHC-F - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
- Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
- Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
- Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Rouers A, et al. 2021. Cell Rep Med. 2:100278. PubMed
- Martin–Fernandez M, et al. 2020. Cell Reports. 31(6):107633. PubMed
- Meckiff BJ, et al. 2019. J Immunol. 203:1276. PubMed
- Ehling RA, et al. 2022. Cell Rep. 38:110242. PubMed
- Taft J, et al. 2021. Cell. 184(17):4447-4463.e20. PubMed
- Brinkmann CR, et al. 2018. mSphere. 3:e00616. PubMed
- Wang W, et al. 2018. Cancer Cell. 34:757. PubMed
- Kitai Y, et al. 2017. J Immunol. 198:1649. PubMed
- Guo T, et al. 2018. J Immunol. 200:500. PubMed
- Martin JC, et al. 2020. Cell. 178(6):1493-1508.e20.. PubMed
- Gruber CN, et al. 2020. Cell. 183:982. PubMed
- O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
- Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed
- Martins M, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006529. PubMed
- Patel NP, et al. 2018. J Infect Dis. 218:S88. PubMed
- Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
- Sattler A, et al. 2021. Am J Transplant. 21:87. PubMed
- Yang Q, et al. 2020. J Immunol. 2331:204. PubMed
- Chng MHY, et al. 2020. Immunity. 51(6):1119-1135.e5.. PubMed
- Ardain A, et al. 2019. Nature. 570:528. PubMed
- Singh AK, et al. 2017. Front Immunol. 0.513888889. PubMed
- Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
- NULL, et al. 2022. Cell. 185:916. PubMed
- Matos TR, et al. 2017. J Clin Invest. 127:4031. PubMed
- Jones N, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01516. PubMed
- Chiou SH, et al. 2021. Immunity. 54:586. PubMed
- RRID
- AB_314837 (BioLegend Cat. No. 310902)
- Structure
- C-type lectin, type II glycoprotein, 28/32 kD
- Distribution
-
Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
- Function
- Lymphocyte, monocyte, and platelet activation, NK cell killing
- Cell Type
- B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.
- Gene ID
- 969 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org
