PerCP/Cyanine5.5 anti-human CD69 Antibody
- 产品名称：
- PerCP/Cyanine5.5 anti-human CD69 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 310925
- 产品应用：
- 310925
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2112 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and 564 nm and a maximun emission of 690 nm.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.
- Additional Product Notes
- BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
- Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
- Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
- Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Lutwama F, et al. 2014. J Infect Dis. 209:887. PubMed
- de Jonge K et al. 2019. Scientific reports. 9(1):4487 . PubMed
- Tatovic D, et al. 2015. J Immunol. 195: 386 - 392. PubMed
- Walk J, et al. 2019. Nat Commun. 10:874. PubMed
- Jeyanathan M, et al. 2022. JCI Insight. 7:. PubMed
- Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
- Danzer H, et al. 2020. Elife. 9:00. PubMed
- Vlková M, et al. 2010. J Immunol. 185:6431. PubMed
- Trinité B, et al. 2014. PLoS One. 9:110719. PubMed
- Harris LD, et al. 2020. Front Cell Infect Microbiol. 10:120. PubMed
- RRID
- AB_2074957 (BioLegend Cat. No. 310925) AB_2074956 (BioLegend Cat. No. 310926)
- Structure
- C-type lectin, type II glycoprotein, 28/32 kD
- Distribution
-
Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
- Function
- Lymphocyte, monocyte, and platelet activation, NK cell killing
- Cell Type
- B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.
- Gene ID
- 969 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org
