PE anti-human CD69 Antibody
- 产品名称：
- PE anti-human CD69 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 310905
- 产品应用：
- 310905
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The CD69 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
- Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
- Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
- Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
- Kongsomboonvech AK, et al. 2020. PLoS Pathog. 16:e1008327. PubMed
- Marquardt I, et al. 2022. Front Microbiol. 12:752549. PubMed
- Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
- Magalh?es L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
- Kieffer T, et al. 2017. J Reprod Immunol. 10.1016/j.jri.2016.11.004. PubMed
- Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
- Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
- Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
- Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
- Fiaux PC, et al. 2020. PLoS Comput Biol. 16:e1008194. PubMed
- Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
- Park S, et al. 2015. J Biol Chem. 290: 17349 - 17366. PubMed
- Thakral D, et al. 2008. J Immunol. 180:7431. PubMed
- Bellini N, et al. 2022. iScience. 25:105234. PubMed
- Oikawa D, et al. 2020. Front Immunol. 11:601926. PubMed
- Stuart T, et al. 2019. Cell. 177:1888. PubMed
- Gardner TJ, et al. 2022. Nat Chem Biol. 18:216. PubMed
- Lucas C, et al. 2021. Nature. Online ahead of print. PubMed
- Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
- Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
- Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
- Jaudszus A, et al. 2013. J Lipid Res. 54:923. PubMed
- Arvindam US, et al. 2021. Leukemia. 35:1586. PubMed
- Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed
- Zhou Y, et al. 2016. J Leukoc Biol. 100: 1201 - 1211. PubMed
- Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
- Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
- Wallstabe L et al. 2019. JCI Insight. 4(18) pii: 126345. PubMed
- Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed
- Costantini C, et al. 2010. Int Immunol. 1.490972222. PubMed
- Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed
- Liu Y, et al. 2022. MAbs. 14:2073632. PubMed
- Feng Y, et al. 2022. Life (Basel). 12:. PubMed
- Li W, et al. 2020. Immunity. 53(2):456-470. PubMed
- Zhang P, et al. 2021. Nat Med. 22:. PubMed
- Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed
- Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
- Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
- Zhou Z, et al. 2022. J Gastrointest Oncol. 13:732. PubMed
- Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
- Su S, et al. 2016. Sci Rep. 6:20070. PubMed
- Wang R, et al. 2016. Proc Natl Acad Sci U S A. 113: 11501 - 11506. PubMed
- Cohen Z, et al. 2020. Biomed Rep. 13:17. PubMed
- Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
- Zimmermann M, et al. 2019. Front Immunol. 10:829. PubMed
- Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
- Shen C, et al. 2021. BMC Med. 19:283. PubMed
- Hoseini SS, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
- Guenther C, et al. 2019. Front Immunol. 10:1138. PubMed
- Shan L, et al. 2017. Immunity. 47:766. PubMed
- RRID
- AB_314840 (BioLegend Cat. No. 310905) AB_314841 (BioLegend Cat. No. 310906)
- Structure
- C-type lectin, type II glycoprotein, 28/32 kD
- Distribution
-
Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
- Function
- Lymphocyte, monocyte, and platelet activation, NK cell killing
- Cell Type
- B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.
- Gene ID
- 969 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org
