Pacific Blue™ anti-human CD69 Antibody
- 产品名称：
- Pacific Blue™ anti-human CD69 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 310919
- 产品应用：
- 310919
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
- Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
- Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
- Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Howson LJ, et al. 2018. Nat Commun. 9:253. PubMed
- Rodriguez-García A, et al. 2020. Mol Ther. 28:548. PubMed
- Panciera T, et al. 2016. Cell Stem Cell. 19:725-737. PubMed
- Lee JM, et al. 2022. Lupus Sci Med. 9:. PubMed
- Jan M, et al. 2021. Sci Transl Med. 13:. PubMed
- Francescone R, et al. 2020. Cancer Discovery. 11(2):446-479. PubMed
- Verma K, et al. 2017. PLoS One.. 10.1371/journal.pone.0183828. PubMed
- Forster F, et al. 2014. J Immunol. 192:771. PubMed
- Kathamuthu GR, et al. 2021. Front Cell Infect Microbiol. 11:756854. PubMed
- Chen EW, et al. 2019. Front Immunol. 10:1718. PubMed
- Tsai C, et al. 2015. J Immunol. 194:3890. PubMed
- RRID
- AB_493666 (BioLegend Cat. No. 310919) AB_493667 (BioLegend Cat. No. 310920)
- Structure
- C-type lectin, type II glycoprotein, 28/32 kD
- Distribution
-
Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
- Function
- Lymphocyte, monocyte, and platelet activation, NK cell killing
- Cell Type
- B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.
- Gene ID
- 969 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org
