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FITC anti-human CD69 Antibody
产品名称:
FITC anti-human CD69 Antibody
产品类别:
抗体
产品编号:
310904
产品应用:
310904
[价格]
规格 价格 库存
100tests ¥ 1284 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  9. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
  2. Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed
  3. Vinogradova EV, et al. 2021. STAR Protoc. 2:100458. PubMed
  4. Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
  5. González-Jiménez A, et al. 2022. Int J Mol Sci. 23:. PubMed
  6. Zhou X, et al. 2021. J Clin Transl Hepatol. 9:283. PubMed
  7. Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
  8. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  9. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  10. Demaret J, et al. 2021. Front Immunol. 12:778679. PubMed
  11. Alkasalias T, et al. 2022. Cell Death Discov. 8:464. PubMed
  12. Robertson N, et al. 2020. J Biol Chem. . PubMed
  13. Tanno H, et al. 2020. Sci Adv. 6:eaay9093. PubMed
  14. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  15. Fosdick MG, et al. 2022. Sci Rep. 12:13506. PubMed
  16. Okoye AA, et al. 2022. PLoS Pathog. 18:e1010245. PubMed
  17. Shifrut E et al. 2018. Cell. 175(7):1958-1971 . PubMed
  18. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  19. Finetti F, et al. 2015. J Cell Sci. 128: 2541 - 2552. PubMed
  20. Vinogradova EV, et al. 2020. Cell. 182(4):1009-1026.e29. PubMed
  21. Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
  22. Cohen O, et al. 2012. Int Immunol. 24:783. PubMed
  23. Ferry GM, et al. 2022. Front Immunol. 13:863155. PubMed
  24. Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
  25. Yukawa M, et al. 2020. J Exp Med. 217:00:00. PubMed
  26. Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
  27. Ledderose C, et al. 2021. J Leukoc Biol. 109:497. PubMed
  28. Cassioli C, et al. 2021. Front Cell Dev Biol. 9:673446. PubMed
  29. Gao R, et al. 2018. Oncoimmunology. 7:e1438108. PubMed
  30. Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
  31. Pettmann J, et al. 2021. eLife. 10:00. PubMed
  32. Robertson N, et al. 2020. J Biol Chem. 295:18436. PubMed
  33. Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
RRID
AB_314838 (BioLegend Cat. No. 310903) AB_314839 (BioLegend Cat. No. 310904)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

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