FITC anti-human CD69 Antibody
- 产品名称：
- FITC anti-human CD69 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 310903
- 产品应用：
- 310903
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.
- Application References
(PubMed link indicates BioLegend citation) -
- Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
- Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
- Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
- Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
- Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Ho CH, et al. 2021. Arthritis Res Ther. 23:199. PubMed
- Cluff E, et al. 2022. Cancer Immunol Immunother. . PubMed
- Vinogradova EV, et al. 2021. STAR Protoc. 2:100458. PubMed
- Nixon CC, et al. 2020. Cell Reports Medicine. 578(7793):160-165. PubMed
- González-Jiménez A, et al. 2022. Int J Mol Sci. 23:. PubMed
- Zhou X, et al. 2021. J Clin Transl Hepatol. 9:283. PubMed
- Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed
- Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
- Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
- Demaret J, et al. 2021. Front Immunol. 12:778679. PubMed
- Alkasalias T, et al. 2022. Cell Death Discov. 8:464. PubMed
- Robertson N, et al. 2020. J Biol Chem. . PubMed
- Tanno H, et al. 2020. Sci Adv. 6:eaay9093. PubMed
- Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
- Fosdick MG, et al. 2022. Sci Rep. 12:13506. PubMed
- Okoye AA, et al. 2022. PLoS Pathog. 18:e1010245. PubMed
- Shifrut E et al. 2018. Cell. 175(7):1958-1971 . PubMed
- Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
- Finetti F, et al. 2015. J Cell Sci. 128: 2541 - 2552. PubMed
- Vinogradova EV, et al. 2020. Cell. 182(4):1009-1026.e29. PubMed
- Rydyznski Moderbacher C, et al. 2020. Cell. 183(4):996-1012.e19. PubMed
- Cohen O, et al. 2012. Int Immunol. 24:783. PubMed
- Ferry GM, et al. 2022. Front Immunol. 13:863155. PubMed
- Fang F, et al. 2021. Cell Rep. 37:109981. PubMed
- Yukawa M, et al. 2020. J Exp Med. 217:00:00. PubMed
- Rydyznski Moderbacher C, et al. 2022. J Clin Invest. :. PubMed
- Ledderose C, et al. 2021. J Leukoc Biol. 109:497. PubMed
- Cassioli C, et al. 2021. Front Cell Dev Biol. 9:673446. PubMed
- Gao R, et al. 2018. Oncoimmunology. 7:e1438108. PubMed
- Lavaert M, et al. 2020. Frontiers in Immunology. 1.610416667. PubMed
- Pettmann J, et al. 2021. eLife. 10:00. PubMed
- Robertson N, et al. 2020. J Biol Chem. 295:18436. PubMed
- Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
- RRID
- AB_314838 (BioLegend Cat. No. 310903) AB_314839 (BioLegend Cat. No. 310904)
- Structure
- C-type lectin, type II glycoprotein, 28/32 kD
- Distribution
-
Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
- Function
- Lymphocyte, monocyte, and platelet activation, NK cell killing
- Cell Type
- B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.
- Gene ID
- 969 View all products for this Gene ID
- UniProt
- View information about CD69 on UniProt.org
