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APC/Cyanine7 anti-human CD69 Antibody
产品名称:
APC/Cyanine7 anti-human CD69 Antibody
产品类别:
抗体
产品编号:
310913
产品应用:
310913
[价格]
规格 价格 库存
25tests ¥ 1860 1

产品详情

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
  9. Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
Product Citations
  1. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  2. Braun J, et al. 2020. Nature. 587:270. PubMed
  3. Ahmed R et al. 2019. Cell. 177(6):1583-1599 . PubMed
  4. Sorensen ES, et al. 2020. Antimicrob Agents Chemother. 64:00:00. PubMed
  5. Koelsch K, et al. 2013. PLoS One. 8:50068. PubMed
  6. Di Blasi D, et al. 2019. Cell Mol Gastroenterol Hepatol. 0.510416667. PubMed
  7. Holbrook AK, et al. 2019. Physiol Rep. 7:e14234. PubMed
  8. Judge CJ, et al. 2017. J Leukoc Biol. 102:171. PubMed
  9. Fisher J, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.03.002. PubMed
  10. Yin Q, et al. 2021. Proc Natl Acad Sci U S A. 118: . PubMed
  11. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  12. Fu W, et al. 2016. Sci Rep. 6:38162. PubMed
  13. Pallikkuth S, et al. 2020. eLife. 9:e51889.. PubMed
  14. Zhu Y, et al. 2019. Cell Stem Cell. 25:542. PubMed
  15. Li YR, et al. 2021. Cell Rep Med. 2:100449. PubMed
  16. Saraiva DP, et al. 2018. Front Immunol. 2.184027778. PubMed
  17. Arenas EJ, et al. 2021. Nat Commun. 12:1237. PubMed
  18. Paulikat AD, et al. 2022. J Innate Immun. . PubMed
  19. Qi Y, et al. 2012. PLoS One. 7:e39072. PubMed
  20. Hughson A, et al. 2011. Diabetes. 60:2125. PubMed
  21. Lichnog C, et al. 2019. Front Pharmacol. 10:39. PubMed
  22. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  23. Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed
  24. Corgnac S, et al. 2022. J Immunother Cancer. 10:. PubMed
  25. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
  26. Schreurs RRCE, et al. 2021. Mucosal Immunology. 14(3):605-614. PubMed
  27. Corgnac S, et al. 2021. STAR Protocols. 2(1):100267. PubMed
  28. Tsai C, et al. 2015. J Immunol. 194:3890. PubMed
  29. Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
RRID
AB_314848 (BioLegend Cat. No. 310913) AB_314849 (BioLegend Cat. No. 310914)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

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