Alexa Fluor® 647 anti-human CD69 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100tests	￥ 3180	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested
SB -?Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections², immunofluorescence microscopy³, and spatial biology (IBEX)^8,9.

Additional Product Notes

Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).

Application References (PubMed link indicates BioLegend citation)

Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Willcox CR, et al. 2020. Immunity. 51(5):813-825.e4.. PubMed
Greenberg J, et al. 2022. JCI Insight. Online ahead of print. PubMed
Harris MJ, et al. 2021. Mol Syst Biol. 17:e10091. PubMed
Leite NC, et al. 2020. Cell Reports. 32(2):107894.. PubMed
Rasmussen TA, et al. 2022. Cell Rep Med. 3:100766. PubMed
Li Z, et al. 2018. J Immunol. 200:1471. PubMed
Pettmann J, et al. 2021. eLife. 10:00. PubMed

RRID

AB_528871 (BioLegend Cat. No. 310918)

Antigen Details

Structure: C-type lectin, type II glycoprotein, 28/32 kD
Distribution: Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells
Function: Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type: B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.
Gene ID: 969 View all products for this Gene ID
UniProt: View information about CD69 on UniProt.org

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