Ultra-LEAF™ Purified anti-human/mouse integr
- 产品名称：
- Ultra-LEAF™ Purified anti-human/mouse integr
- 产品类别：
- 抗体
- 产品编号：
- 321233
- 产品应用：
- 321233
- Verified Reactivity
- Human, Mouse, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TK1 cells
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The FIB504 antibody has been reported to react with mouse and human ?7 integrin and to block ?7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF? purified FIB504 antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 321233-321238).
- Application References
(PubMed link indicates BioLegend citation) -
- Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
- Berlin C, et al. 1993. Cell 74:185.
- Rott LS, et al. 1996. J. Immunol. 156:3727. (Block)
- Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block)
- Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- RRID
- AB_2832628 (BioLegend Cat. No. 321233) AB_2832629 (BioLegend Cat. No. 321234) AB_2860840 (BioLegend Cat. No. 321235) AB_2860841 (BioLegend Cat. No. 321236) AB_2860842 (BioLegend Cat. No. 321237) AB_2876624 (BioLegend Cat. No. 321238)
- Structure
- Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
- Distribution
-
CD49d/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes
- Function
- Lymphocyte adhesion, hematopoietic progenitor cells migration
- Ligand/Receptor
- CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Picarella D, et al. 1997. J. Immunol. 158:2099.
3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635
4. Cepek KL, et al. 1994. Nautre 372:190.
- Gene ID
- 16421 View all products for this Gene ID 3695 View all products for this Gene ID
- UniProt
- View information about Integrin beta7 on UniProt.org
