FITC anti-human/mouse Integrin β7 Antibody
- 产品名称：
- FITC anti-human/mouse Integrin β7 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 321213
- 产品应用：
- 321213
- Verified Reactivity
- Human, Mouse, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon, Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- TK1 cells
- Formulation
- test size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
?g sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. - Preparation
- The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
- Concentration
- ?g sizes: 0.5 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the test size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g sizes, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
- Application Notes
The FIB504 antibody has been reported to react with mouse and human ?7 integrin and to block ?7 integrin-mediated cell adhesion in in vitro and in vivo studies. Additional reported applications (for the relevant formats) include: blocking of cell adhesion1,3,4. The Ultra-LEAF? purified FIB504 antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 321233-321238).
- Application References
(PubMed link indicates BioLegend citation) -
- Andrew DP, et al. 1994. J. Immunol. 153:3847. (Block)
- Berlin C, et al. 1993. Cell 74:185.
- Rott LS, et al. 1996. J. Immunol. 156:3727. (Block)
- Rivera-Nieves J, et al. 2005. J. Immunol. 174:2343. (Block)
- Ohmori K, et al. 2009. J. Immunol. 182:2835. PubMed
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Product Citations
-
- Townsley SM, et al. 2021. Cell Host Microbe. 29(4):564-578.e9. PubMed
- R?hl M, et al. 2021. Vaccines (Basel). 9:. PubMed
- Chu C, et al. 2013. PLoS One. 8:64187. PubMed
- Sato T, et al. 2020. Sci Rep. 10:13221. PubMed
- Ohmori K, et al. 2009. J Immunol. 182:2835. PubMed
- Durant L, et al. 2020. Microbiome. 8:88. PubMed
- Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
- Townsley SM, et al. 2021. STAR Protocols. 2(3):100771. PubMed
- RRID
- AB_830857 (BioLegend Cat. No. 321213) AB_830856 (BioLegend Cat. No. 321212) AB_830858 (BioLegend Cat. No. 321214)
- Structure
- Integrin family, Ig superfamily, 130 kD, heterodimer with CD49d or CD103
- Distribution
-
CD49d/β7: majority of lymphocytes, thymocyte subset, and some bone marrow progenitors; CD103/β7: intestinal intraepithelial lymphocytes (IEL), dendritic epidermal T cells, subset of CD8 T cells in lymph nodes
- Function
- Lymphocyte adhesion, hematopoietic progenitor cells migration
- Ligand/Receptor
- CD49d/β7: VCAM-1 (CD106), MAdCAM-1 and fibronectin; CD103/β7: E-cadherin
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, Lymphocytes, T cells, Thymocytes, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
1. Andrew DP, et al. 1994. J. Immunol. 153:3847.
2. Picarella D, et al. 1997. J. Immunol. 158:2099.
3. Lefrancois L, et al. 1994. Eur. J. Immunol. 24:635
4. Cepek KL, et al. 1994. Nautre 372:190.
- Gene ID
- 16421 View all products for this Gene ID 3695 View all products for this Gene ID
- UniProt
- View information about Integrin beta7 on UniProt.org
