PE anti-mouse CD40 Antibody
- 产品名称：
- PE anti-mouse CD40 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 157505
- 产品应用：
- 157505
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant mouse CD40 – Fc fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The FGK45 antibody can block the binding of HM40-3 clone. The FGK45 antibody can also stimulate mouse B cell proliferation.
- Application References
(PubMed link indicates BioLegend citation) -
- Andersson J, et al. 1996. Immunity 5:319-330.
- Melief CJ, et al. 1998. Nature 393(6684):480-3.
- Londei M, et al. 2000. Nat Med 6(6):673-9.
- Tominaga T, et al. 2015. Neuro Oncol 17(11):1453-62.
- Product Citations
-
- Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
- RRID
- AB_2832552 (BioLegend Cat. No. 157505) AB_2860731 (BioLegend Cat. No. 157506)
- Structure
- 48 kD type I transmembrane glycoprotein, a member of the tumor necrosis factor receptor (TNFR) superfamily.
- Distribution
-
Expressed on B lymphocytes and other antigen-presenting cells, such as macrophages, follicular dendritic cells, etc.
- Function
- Regulates B cell growth and differentiation and Ig isotype switching. Interaction of CD40 with its ligand, CD154, on activated T cells is important for immune response.
- Interaction
- TRAF molecules
- Ligand/Receptor
- CD154 (CD40L)
- Cell Type
- Antigen-presenting cells, B cells, Dendritic cells, Macrophages
- Biology Area
- Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules
- Antigen References
-
- Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
- Bancherou J, et al. 1994. Annu. Rev. Immunol. 12:881.
- Clark EA, et al. 1996. P. Natl. Acad. Sci. USA 83:4494.
- Grewal IS, et al. 1998. Annu Rev Immunol 16:111.
- Gene ID
- 21939 View all products for this Gene ID
- UniProt
- View information about CD40 on UniProt.org
