Purified anti-GAPDH Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1488	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human CD4 lymphocytes

Formulation

This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

Upon receipt, store undiluted between 2°C and 8°C.

Application

WB - Quality tested
IHC, IP - Reported by the developer, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 0.1 - 1.0 ?g antibody per 1 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.
Clone FF26A/F9 is only reactive to human GAPDH, and not cross-reactive to mouse or rat GAPDH.

Application References (PubMed link indicates BioLegend citation)

Maestre L, et al. 2009. Haematologica. 94:419.
Zou L, et al. 2012. J Biol. Chem. 287:7190. PubMed
Chen CY, et al. 2012. PNAS. 110:630 PubMed
Trifari S, et al. 2013. PNAS. PubMed
Liu CC, et al. 2013. Mol. Cell Biol. 33:4334. PubMed
Trifari S, et al. 2013. PNAS. 110:18608. PubMed
Butin-Israeli V, et al. 2015. Mol. Cell Biol. 35:884. PubMed

Product Citations

Trifari S, et al. 2013. Proc Natl Acad Sci U S A. 110:18608. PubMed
Wahlig S, et al. 2020. Sci Rep. 1.584027778. PubMed
Ong SM, et al. 2018. Cell Death Dis. 9:266. PubMed
Zeng L, et al. 2021. J Cell Biol. 220:. PubMed
Zou L, et al. 2012. J Biol Chem. 287:7190. PubMed
Butin-Israeli V, et al. 2015. Mol Cell Biol. 35:884. PubMed
Ruehle S et al. 2017. Journal of neurochemistry. 142(4):521-533 . PubMed
Liu R, et al. 2021. Oncol Lett. 21:06. PubMed
Liu C, et al. 2013. Mol Cell Biol. 33:4334. PubMed
Betriu N, et al. 2021. Cancers (Basel). 13:. PubMed
Gorji-Bahri G, et al. 2021. J Cell Biochem. 122:425. PubMed
Lovatt M, et al. 2018. Antioxidants (Basel). 0.416666667. PubMed
Barbosa JAF, et al. 2021. J Virol. 95:e0097120. PubMed
Chen C, et al. 2013. Proc Natl Acad Sci U S A. 110:630. PubMed
Zhao J et al. 2019. Transl Psychiatry. 9(1):247 . PubMed
Dragoj M, et al. 2017. Invest New Drugs. 10.1007/s10637-017-0494-4. PubMed

RRID

AB_10613283 (BioLegend Cat. No. 649201) AB_10612752 (BioLegend Cat. No. 649202)

Antigen Details

Structure: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution: Ubiquitously expressed, locate in cytoplasm and perinuclear region.
Function: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and and nicotinamide adenine dinucleotide (NAD). Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretion pathway.
Interaction: Interacts with TPPP. Interacts with EIF1ADand WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Signal Transduction
Antigen References: 1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
2. Meyer-Siegler K, et al. 1991. P. Natl. Acad. Sci. USA 88:8460.
3. Hara MR and Snyder SH. 2006. Cell Mol. Neurobiol. 26:527.
4. Zheng L, et al. 2003. Cell. 114:255.
5. Wang Q, et al. 2005. FASEB J. 19:869.
6. Bae BI, et al. 2006. P. Natl. Acad. Sci. USA. 103:3405.
Gene ID: 5590 View all products for this Gene ID
UniProt: View information about GAPDH on UniProt.org

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