Direct-Blot™ HRP anti-GAPDH Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µL	￥ 1212	1

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

Human CD4 lymphocytes

Formulation

This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.

Preparation

The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)

Storage & Handling

Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.

Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution is 1:1000-1:2000. The optimal dilution should be determined by titration for each individual assay of interest.

25 ?l and 100 ?l of Direct-Blot? HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

The optimal concentration should be determined by titration for each individual assay of interest.
Clone FF26A/F9 is only reactive to human GAPDH, and not cross-reactive to mouse or rat GAPDH.

Application References (PubMed link indicates BioLegend citation)

Maestre L, et al. 2009. Haematologica. 94:419.
Zou L, et al. 2012. J Biol. Chem. 287:7190. PubMed
Chen CY, et al. 2012. PNAS. 110:630 PubMed
Trifari S, et al. 2013. PNAS. PubMed
Liu CC, et al. 2013. Mol. Cell Biol. 33:4334. PubMed
Trifari S, et al. 2013. PNAS. 110:18608. PubMed
Butin-Israeli V, et al. 2015. Mol. Cell Biol. 35:884. PubMed

Product Citations

Kyle Cottrell, Sergej Djuranovic 2016. PLoS One. 11:e0167877. PubMed
Wang Z, et al. 2019. Sci Rep. 9:1853. PubMed
Powell G, et al. 2021. Mol Ther Nucleic Acids. 26:865. PubMed
Zu T, et al. 2021. Lab Invest. 101:636. PubMed

RRID

AB_2734517 (BioLegend Cat. No. 649204) AB_2566698 (BioLegend Cat. No. 649203)

Antigen Details

Structure: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family, predicted molecular weight 36 kD. The enzyme exists as a tetramer of identical chains.
Distribution: Ubiquitously expressed, locate in cytoplasm and perinuclear region.
Function: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and and nicotinamide adenine dinucleotide (NAD). Independent of its glycolytic activity it is also involved in membrane trafficking in the early secretion pathway.
Interaction: Interacts with TPPP. Interacts with EIF1ADand WARS. Interacts with SUMO4, GLUT4, nPKC-iota and CAMK2
Biology Area: Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Signal Transduction, Western Blot Controls
Antigen References: 1. Ercolani L, et al. 1988. J. Biol. Chem. 263:15335.
2. Meyer-Siegler K, et al. 1991. P. Natl. Acad. Sci. USA 88:8460.
3. Hara MR and Snyder SH. 2006. Cell Mol. Neurobiol. 26:527.
4. Zheng L, et al. 2003. Cell. 114:255.
5. Wang Q, et al. 2005. FASEB J. 19:869.
6. Bae BI, et al. 2006. P. Natl. Acad. Sci. USA. 103:3405.
Gene ID: 5590 View all products for this Gene ID
UniProt: View information about GAPDH on UniProt.org

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