Spark YG™ 570 anti-mouse CD68 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1836	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Purified Con A receptor glycoproteins from the P815 cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide

Preparation

The antibody was purified by affinity chromatography and conjugated with Spark YG? 570 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.25?- 5.0??g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark YG? 570 has a maximum excitation of 555 nm and a maximum emission of 570 nm.

Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation^{1, 2}, Western Blot^{1, 2},?immunohistochemical staining of frozen sections² and paraformaldehyde-fixed paraffin-embedded sections³, and spatial biology (IBEX)^9,10.

Application References (PubMed link indicates BioLegend citation)

Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
Kayama H, et al. 2012. PNAS. 109:5010. PubMed
Park S, et al. 2013. Biomaterials. 34:598. PubMed
Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

RRID

AB_2876476 (BioLegend Cat. No. 137037) AB_2876476 (BioLegend Cat. No. 137038)

Antigen Details

Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References: 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.
Gene ID: 12514 View all products for this Gene ID
UniProt: View information about CD68 on UniProt.org

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