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首页 > 抗体蛋白 > 抗体
Purified anti-mouse CD68 Antibody
产品名称：
Purified anti-mouse CD68 Antibody
产品类别：
抗体
产品编号：
137001
产品应用：
137001
[价格]
规格 价格 库存
50µg ￥ 1212 1

产品详情

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Purified Con A receptor glycoproteins from the P815 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICFC - Quality tested
FC - Verified
IP, WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation1, 2, Western Blot1, 2,?immunohistochemical staining of frozen sections2 and paraformaldehyde-fixed paraffin-embedded sections3, and spatial biology (IBEX)9,10.

Application References

(PubMed link indicates BioLegend citation)
  1. Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
  2. Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
  3. Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
  4. Kayama H, et al. 2012. PNAS. 109:5010. PubMed
  5. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  6. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  7. McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
  8. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  9. Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
  10. Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed
Product Citations
  1. Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
  2. Zaman R, et al. 2021. Immunity. :. PubMed
  3. Fang W, et al. 2020. Transl Res. 222:28. PubMed
  4. Milasan A, et al. 2017. J Am Heart Assoc. 10.1161/JAHA.117.006892. PubMed
  5. Filipello F et al. 2018. Immunity. 48(5):979-991 . PubMed
  6. Miho Sato‐Hashimoto et al. 2019. eLife. 8 pii: e42025. PubMed
  7. Preda MB, et al. 2021. Cell Death Dis. 12:566. PubMed
  8. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  9. Mathew DJ, et al. 2021. Sci Rep. 11:22880. PubMed
  10. Da Mesquita S, et al. 2021. Nature. 593:255. PubMed
  11. McKinstry S, et al. 2014. J Neurosci. 34:9455. PubMed
  12. Spencer WJ, et al. 2019. Proc Natl Acad Sci U S A. 116:13087. PubMed
  13. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  14. Guimbal S, et al. 2021. Arterioscler Thromb Vasc Biol. 41:e193. PubMed
  15. Park S, et al. 2013. Biomaterials. 34:598. PubMed
  16. Henjum K, et al. 2020. Sci Rep. 10:10590. PubMed
  17. Sherafat A, et al. 2021. Nat Commun. 12:2265. PubMed
  18. Gomez-Pastor R, et al. 2017. Nat Commun. 8:14405. PubMed
  19. Trus I, et al. 2020. Front Immunol. 2.553472222. PubMed
  20. Shen L et al. 2019. Br J Pharmacol. 176(14):2559-2572 . PubMed
  21. Tian K, et al. 2021. J Immunol Res. 2021:6297332. PubMed
  22. Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
  23. Brocker CN, et al. 2017. Am J Physiol Gastrointest Liver Physiol. 312:G283. PubMed
  24. Di Gioia M, et al. 2020. Nat Immunol. 21:42. PubMed
  25. Burrus CJ, et al. 2020. Cell Rep. 30:642. PubMed
  26. Batool M, et al. 2020. Basic Res Cardiol. 115:68. PubMed
  27. Mariot V, et al. 2021. J Cachexia Sarcopenia Muscle. Online ahead of print. PubMed
  28. O'Dea KP, et al. 2020. J Extracell Vesicles. 9:1706708. PubMed
  29. Varol D et al. 2017. Immunity. 46(6):1030-1044 . PubMed
  30. Mori H, et al. 2015. Toxicol Pathol. 43: 883-889. PubMed
  31. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  32. Silburt J, et al. 2022. J Neuroinflammation. 19:24. PubMed
  33. Kim H, et al. 2020. ACS Nano. 14:6519. PubMed
  34. Goldblatt DL, et al. 2020. British Journal of Pharmacology. 177(10):2256-2273.. PubMed
  35. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  36. Tsubaki T, et al. 2018. Oncotarget. 9:11209. PubMed
  37. Carvalho K, et al. 2022. J Neuroinflammation. 19:178. PubMed
  38. Stafeev I, et al. 2022. Front Nutr. 8:809732. PubMed
  39. Shi W, et al. 2021. Biosci Rep. 41:. PubMed
  40. Godoy-Calderón MJ, et al. 2018. Oncotarget. 8:11370. PubMed
  41. Wu Y, et al. 2019. Nat Commun. 10:58. PubMed
  42. Cornelison RC, et al. 2022. NPJ Precis Oncol. 6:54. PubMed
  43. Wang L, et al. 2020. Sci Rep. 10:16326. PubMed
  44. Collier AF, et al. 2018. J Pharmacol Exp Ther. 364:409. PubMed
  45. Choi SH, et al. 2020. Arterioscler Thromb Vasc Biol. :ATVBAHA120315485. PubMed
  46. Cushing EM, et al. 2018. J Lipid Res. 59:1230. PubMed
  47. Ikezoe T, et al. 2021. Biomolecules. 11:. PubMed
  48. Koch PS, et al. 2021. Front Physiol. 12:722394. PubMed
  49. Phua T, et al. 2017. Sci Rep. 10.1038/srep44351. PubMed
  50. Cheng X, et al. 2021. Redox Biol. 40:101863. PubMed
  51. Milasan A, et al. 2019. Atherosclerosis. 283:106. PubMed
  52. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  53. Tsai MS, et al. 2021. Int J Mol Sci. 22:. PubMed
  54. Ni K, et al. 2018. Respir Res. 0.865972222. PubMed
  55. Dong Y, et al. 2021. STAR Protoc. 2:100853. PubMed
  56. Yue F, et al. 2021. Mol Ther. 29:132. PubMed
  57. Healy A, et al. 2020. Arterioscler Thromb Vasc Biol. 40:714. PubMed
  58. Kayama H, et al. 2012. Proc Natl Acad Sci U S A. 109:5010. PubMed
  59. Han X, et al. 2020. Mol Carcinog. 59:1088. PubMed
RRID
AB_2044003 (BioLegend Cat. No. 137001) AB_2044004 (BioLegend Cat. No. 137002)

Antigen Details

Structure
A member of the lysosomal-associated membrane protein (lamp) family.
Distribution

Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.

Function
Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type
Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References

1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.

Gene ID
12514 View all products for this Gene ID
UniProt
View information about CD68 on UniProt.org

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