Brilliant Violet 711™ anti-mouse CD68 Antibo-上海雅吉生物科技有限公司

规格	价格	库存
50µg	￥ 3636	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Purified Con A receptor glycoproteins from the P815 cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).

Preparation

The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 711? under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 711? excites at 405 nm and emits at 711 nm. The bandpass filter 710/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 711? is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet?.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.

Excitation Laser

Violet Laser (405 nm)

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation^{1, 2}, Western Blot^{1, 2},?immunohistochemical staining of frozen sections² and paraformaldehyde-fixed paraffin-embedded sections³, and spatial biology (IBEX)^9,10.

Application References (PubMed link indicates BioLegend citation)

Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
Kayama H, et al. 2012. PNAS. 109:5010. PubMed
Park S, et al. 2013. Biomaterials. 34:598. PubMed
Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Di Campli MP, et al. 2020. Eur Respir J. . PubMed

RRID

AB_2783098 (BioLegend Cat. No. 137029)

Antigen Details

Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References: 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.
Gene ID: 12514 View all products for this Gene ID
UniProt: View information about CD68 on UniProt.org

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