APC/Cyanine7 anti-mouse CD68 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 2112	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Purified Con A receptor glycoproteins from the P815 cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.

Concentration

0.2 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested
FC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =0.06 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser

Red Laser (633 nm)

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation^{1, 2}, Western Blot^{1, 2},?immunohistochemical staining of frozen sections² and paraformaldehyde-fixed paraffin-embedded sections³, and spatial biology (IBEX)^9,10.

Additional Product Notes

BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References (PubMed link indicates BioLegend citation)

Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
Kayama H, et al. 2012. PNAS. 109:5010. PubMed
Park S, et al. 2013. Biomaterials. 34:598. PubMed
Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Giorgetti E, et al. 2020. Cell Reports. 29(6):1539-1554.e7.. PubMed
Mao FY, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:395. PubMed
Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed

RRID

AB_2616812 (BioLegend Cat. No. 137023) AB_2616813 (BioLegend Cat. No. 137024)

Antigen Details

Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References: 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.
Gene ID: 12514 View all products for this Gene ID
UniProt: View information about CD68 on UniProt.org

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