Alexa Fluor® 488 anti-mouse CD68 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1764	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Purified Con A receptor glycoproteins from the P815 cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICFC - Quality tested
FC, 3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 ?g/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Blue Laser (488 nm)

Application Notes

Additional reported (for relevant formats) applications include: immunoprecipitation^{1, 2}, Western Blot^{1, 2},?immunohistochemical staining of frozen sections² and paraformaldehyde-fixed paraffin-embedded sections³, and spatial biology (IBEX)^9,10.

Application References (PubMed link indicates BioLegend citation)

Silva RP, et al. 1999. Biochem. J. 338:687. (IP, WB)
Rabinowitz SS, et al. 1991. J. Exp. Med. 174:827. (IP, WB, IHC)
Wu J, et al. 2008. P. Natl. Acad. Sci. USA 105:16934. (IHC)
Kayama H, et al. 2012. PNAS. 109:5010. PubMed
Park S, et al. 2013. Biomaterials. 34:598. PubMed
Guiducci C, et al. 2013. J Exp Med. 210:2903. PubMed
McKinstry SU, et al. 2014. J Neurosci. 34:9455. PubMed
Li X, et al. 2015. J Am Heart Assoc. 6:4. PubMed
Radtke?AJ,?et al.?2020.?Proc Natl Acad Sci U S A.?117:33455-65. (SB)?PubMed
Radtke?AJ,?et al.?2022.?Nat Protoc.?17:378-401. (SB)?PubMed

Product Citations

Barbour T, et al. 2016. Kidney Int. 89: 823-832. PubMed
Logan C, Bowen C, and Menko A 2017. Sci Rep. . 10.1038/s41598-017-16456-5. PubMed
Zhou Y, et al. 2019. Differentiation. 109:16:00. PubMed
Wang LH, et al. 2021. Sci Adv. 7: . PubMed
García L, et al. 2015. Exp Biol Med. 240: 904 - 916. PubMed
DeDreu J, et al. 2020. FASEB J. 34:9316. PubMed
Paterson N, et al. 2022. Elife. 11:. PubMed
Lv F, et al. 2022. iScience. 25:103639. PubMed
Vegas A, et al. 2016. Nat Biotechnol. 10.1038/nbt.3462. PubMed
Kawasaki N, et al. 2013. Proc Natl Acad Sci U S A. 110:7826. PubMed
Tang PC, et al. 2022. Adv Sci (Weinh). 9:e2101235. PubMed
Reitinger C, et al. 2022. Front Immunol. 13:970290. PubMed
Li C, et al. 2020. Cancer Immunol Res. 1.030555556. PubMed

RRID

AB_2074847 (BioLegend Cat. No. 137011) AB_2074846 (BioLegend Cat. No. 137012)

Antigen Details

Structure: A member of the lysosomal-associated membrane protein (lamp) family.
Distribution: Expressed on tissue macrophages, Langerhans cells, and at low levels on dendritic cells.
Function: Involved in cell-cell interaction or cell-ligand interaction, still not completely understood.
Cell Type: Antigen-presenting cells, Dendritic cells, Langerhans cells, Leukocytes, Macrophages
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules, Innate Immune Signaling
Antigen References: 1. Ramprasad MP, et al. 1996. Proc. Natl. Acad. Sci. USA 93:14833.
2. Smith MJ, et al. 1987. J. Cell. Sci. 87:113.
Gene ID: 12514 View all products for this Gene ID
UniProt: View information about CD68 on UniProt.org

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