Purified anti-human CD366 (Tim-3) Antibody
- 产品名称：
- Purified anti-human CD366 (Tim-3) Antibody
- 产品类别：
- 抗体
- 产品编号：
- 345001
- 产品应用：
- 345001
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Tim-3 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.
- Application References
(PubMed link indicates BioLegend citation) -
- Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
- Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
- Klibi J, et al 2009. Blood 113:1957. (FC, Block)
- Product Citations
-
- Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
- Liu F, et al. 2020. J Immunother Cancer. 8:. PubMed
- O'Boyle KC, et al. 2020. Methods Mol Biol. 2111:1. PubMed
- Cobb DA, et al. 2022. J Immunother Cancer. 10:. PubMed
- Eastman AJ, et al. 2021. Am J Reprod Immunol. 85:e13352. PubMed
- Han L, et al. 2019. Haematologica. 10.3324/haematol.2018.205534. PubMed
- Gee MH, et al. 2018. Cell. 172:549. PubMed
- Tao J, et al. 2020. J Cell Mol Med. 24:1046. PubMed
- Zhou H, et al. 2017. Leukemia. 31:2065. PubMed
- Ascierto ML, et al. 2017. Clin Cancer Res. 23:3168. PubMed
- RRID
- AB_2116575 (BioLegend Cat. No. 345001) AB_2116574 (BioLegend Cat. No. 345002)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
- Distribution
-
Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells
- Function
- Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
- Ligand/Receptor
- Galectin-9
- Cell Type
- Dendritic cells, Monocytes, T cells, Th1, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.
- Gene ID
- 84868 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
