Brilliant Violet 785™ anti-human CD366 (Tim-
- 产品名称：
- Brilliant Violet 785™ anti-human CD366 (Tim-
- 产品类别：
- 抗体
- 产品编号：
- 345031
- 产品应用：
- 345031
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2712 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human Tim-3 fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
Brilliant Violet 785? excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for relevant formats of this clone) include: costimulation1 (clone 2E2 has been shown to enhance T-cell receptor mediated activation and cytokine secretion) and blocking2,3.
- Application References
(PubMed link indicates BioLegend citation) -
- Hastings WD, et al. 2009. Eur. J. Immunol. 39:2492. (Costim)
- Jones RB, et al. 2008. J. Exp. Med. 205:2763. (Block)
- Klibi J, et al 2009. Blood 113:1957. (FC, Block)
- Product Citations
-
- Gibellini L, et al. 2020. EMBO Mol Med. 12:e13001. PubMed
- Bezverbnaya K, et al. 2021. Cytotherapy. 23:820. PubMed
- Rha MS, et al. 2021. Immunity. 54:44. PubMed
- Eyquem J, et al. 2017. Nature. 543:113-117. PubMed
- Chow MT et al. 2019. Immunity. 50(6):1498-1512 . PubMed
- Amor C, et al. 2020. Nature. 583:127. PubMed
- Vitanza NA, et al. 2021. Nature Medicine. :. PubMed
- Carnevale J, et al. 2022. Nature. 609:174. PubMed
- Kacherovsky N, et al. 2019. Nat Biomed Eng. 0.66875. PubMed
- Schoutrop E, et al. 2022. Oncoimmunology. 11:2093426. PubMed
- Leclercq G, et al. 2022. J Immunother Cancer. 10:. PubMed
- Khuzwayo S, et al. 2021. Front Immunol. 12:631410. PubMed
- Kim Y, et al. 2021. Oncol Lett. 1.022222222. PubMed
- Mathewson ND, et al. 2021. Cell. 184(5):1281-1298.e26. PubMed
- Karlsson J, et al. 2020. Nat Commun. 1.773611111. PubMed
- Choe JH, et al. 2021. Sci Transl Med. 13:. PubMed
- RRID
- AB_2565832 (BioLegend Cat. No. 345031) AB_2565833 (BioLegend Cat. No. 345032)
- Structure
- Transmembrane protein containing immunoglobulin domain and mucin-like domain; can exist as a soluble form lacking mucin and transmembrane domains
- Distribution
-
Activated T cells, preferentially on Th1 cells, monocytes, dendritic cells
- Function
- Plays a role in regulating macrophage activation, T cell apoptosis and immune tolerance
- Ligand/Receptor
- Galectin-9
- Cell Type
- Dendritic cells, Monocytes, T cells, Th1, Tregs
- Biology Area
- Immunology, Inhibitory Molecules
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
1. Hafler DA and Kuchroo V. 2008. J. Exp. Med. 205:2699.
2. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
3. Wang F, et al. 2009. Immunobiology 214:342.
- Gene ID
- 84868 View all products for this Gene ID
- UniProt
- View information about CD366 on UniProt.org
