Ultra-LEAF™ Purified anti-human CD95 (Fas) A
- 产品名称：
- Ultra-LEAF™ Purified anti-human CD95 (Fas) A
- 产品类别：
- 抗体
- 产品编号：
- 305705
- 产品应用：
- 305705
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Apop - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro induction of apoptosis. The Ultra-LEAF? Purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. Nos. 305705 & 305706).
- Application References
(PubMed link indicates BioLegend citation) -
- Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. New York.
- Product Citations
-
- Namgaladze D, et al. 2021. Cancers (Basel). 13:. PubMed
- Baleeiro RB, et al. 2022. Oncoimmunology. 11:2080329. PubMed
- RRID
- AB_2814144 (BioLegend Cat. No. 305705) AB_2814145 (BioLegend Cat. No. 305706)
- Structure
- TNFR superfamily, type I transmembrane protein, 45 kD
- Distribution
-
T cells and B cells, monocytes, neutrophils, expression level upregulated on activated lymphocytes
- Function
- Mediates apoptosis
- Ligand/Receptor
- Fas ligand (CD178)
- Cell Type
- B cells, Lymphocytes, Monocytes, Neutrophils, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Krammer P, et al. 1994. Immunol. Rev. 142:175.
2. Nagata S, et al. 1995. Science 267:1449.
- Gene ID
- 355 View all products for this Gene ID
- UniProt
- View information about CD95 on UniProt.org
