Purified anti-human CD279 (PD-1) (Maxpar® Read-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 1692	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF?, PG - Verified

Recommended Usage

This product is suitable for use with the?Maxpar? Metal Labeling Kits.?For metal labeling using Maxpar? Ready antibodies,?proceed directly to the step to Partially Reduce the Antibody by adding 100 ?l of Maxpar? Ready antibody to 100 ?l of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar? User Guide when conjugating Maxpar? Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3,?immunohistochemical staining of paraformaldehyde fixed frozen sections¹³, and spatial biology (IBEX)^15,16. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF? purified antibodies (Endotoxin <0.01 EU/?g).

Additional Product Notes

Maxpar? is a registered trademark of Standard BioTools Inc.

Application References (PubMed link indicates BioLegend citation)

Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
Velu V, et al. 2007. J. Virol. 81:5819. (FA)
Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed

Product Citations

Sullivan KD, et al. 2021. Cell Reports. 36(7):109527. PubMed
Neidleman J, et al. 2020. Elife. 9:00. PubMed
Neidleman J, et al. 2020. Cell Rep Med. 100081:1. PubMed
Gide TN, et al. 2019. Cancer Cell. 35:238. PubMed
Galbraith MD, et al. 2021. eLife. 10:00. PubMed
Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
Neidleman J, et al. 2021. Elife. 10:. PubMed
Neidleman J, et al. 2021. Cell Rep. 36:109414. PubMed
Mishra A, et al. 2021. Cell. 184(13):3394-3409.e20. PubMed
Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
Loo Yau H, et al. 2021. Molecular Cell. 81(7):1469-1483.e8. PubMed

RRID

AB_2563734 (BioLegend Cat. No. 329941)

Antigen Details

Structure: Immunoglobulin superfamily
Distribution: Transiently expressed on CD4^- CD8^- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells
Ligand/Receptor: B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Gene ID: 5133 View all products for this Gene ID
UniProt: View information about CD279 on UniProt.org

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