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PerCP/Cyanine5.5 anti-mouse/rat CD81 Antibody
产品名称:
PerCP/Cyanine5.5 anti-mouse/rat CD81 Antibody
产品类别:
抗体
产品编号:
104911
产品应用:
104911
[价格]
规格 价格 库存
25µg ¥ 2112 1

产品详情

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CD81+ mouse B lymphoma 38C13
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

The Eat-2 antibody reacts with mouse and rat CD81. Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, induction of homotypic adhesion of B lymphocytes1, stimulation of B cells undergo early apoptotic events1, and promotion of T cell motility2.?

Additional Product Notes

BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.

Application References

(PubMed link indicates BioLegend citation)
  1. Maecker HT, et al. 2000. Hybridoma 19:15. (IP, WB, Stim)
  2. Clark KL, et al. 2001. J. Immunol. 167:5115. (IP, WB, Activ)
  3. Bhatnagar S and Schorey JS. 2007. J. Biol. Chem. doi:10.1074/jbc.M702277200. PubMed
  4. Castro-Seoane R, et al. 2012. PLoS Pathog. 8:1002538. PubMed
Product Citations
  1. Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed
  2. Wuggenig P, et al. 2020. Commun Biol. 3:130. PubMed
RRID
AB_2562994 (BioLegend Cat. No. 104911) AB_2562995 (BioLegend Cat. No. 104912)

Antigen Details

Structure
TM4SF tetraspanin, 26 kD
Distribution

T cells and B cells, NK cells, dendritic cells, thymocytes, endothelial cells, fibroblasts

Function
Regulates cell growth
Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, NK cells, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Levy S, et al. 1998. Annu. Rev. Immunol. 16:89.
3. Maeker HT, et al. 1997. FASEB J. 11:428.
4. Boismenu R, et al. 1996. Science 271:198.

Gene ID
12520 View all products for this Gene ID 25621 View all products for this Gene ID
UniProt
View information about CD81 on UniProt.org

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