Purified anti-E2F1 Antibody
- 产品名称:
- Purified anti-E2F1 Antibody
- 产品类别:
- 抗体
- 产品编号:
- 941101
- 产品应用:
- 941101
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full-length recombinant human E2F1 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Verified
- Recommended Usage
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 ?g/mL. For immunoprecipitation, the suggested use of this reagent is 2.0 ?g/test. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This clone was tested for ICC using HeLa cells fixed with 4% paraformaldehyde and permeabilized with either methanol or Triton X-100. Neither method resulted in E2F1 staining.
This clone does not recognize mouse E2F1. However, when tested by western blot on mouse lysates, a ~35 kD protein of unknown origin was observed.
- RRID
- AB_2888772 (BioLegend Cat. No. 941101) AB_2888772 (BioLegend Cat. No. 941102)
- Structure
- E2F1 is a 437 amino acid protein with a predicted molecular weight of 47 kD.
- Distribution
-
Ubiquitously expressed/Nucleus
- Function
- Transcription factor/Cell cycle
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Cell Death, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Tumor Suppressors
- Antigen References
-
- Denechaud P,?et al.?2017.?Front. Endocrinol.?8:311.
- Liang Y,?et al.?2015.?Int. J. Oncol.?48:1650.
- Martinez-Balbas M,?et al.?2000.?Embo J.?19:662.
- Wu Z,?et al.?2009.?Int. J. Biochem. Cell Biol.?41:2389.
- Gene ID
- 1869 View all products for this Gene ID
- UniProt
- View information about E2F1 on UniProt.org