Purified anti-mouse CD28 Antibody
- 产品名称：
- Purified anti-mouse CD28 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 122002
- 产品应用：
- 122002
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A20 cells and recombinant mCD28Ig
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 122004).
- Application References
(PubMed link indicates BioLegend citation) -
- Dennehy KM, et al. 2006. J. Immunol. 176:5725.
- Product Citations
-
- Ecker M, et al. 2022. Elife. 11:. PubMed
- Ciraolo E, et al. 2022. Int J Mol Sci. 23:. PubMed
- Kinosada H, et al. 2017. PLoS Pathog. 10.1371/journal.ppat.1006120. PubMed
- RRID
- AB_604072 (BioLegend Cat. No. 122002)
- Structure
- Ig superfamily, 44 kD
- Distribution
-
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
- Function
- Costimulates T and NK cells
- Ligand/Receptor
- CD80 (B7-1), CD86 (B7-2)
- Cell Type
- NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.
- Gene ID
- 12487 View all products for this Gene ID
- UniProt
- View information about CD28 on UniProt.org
