Alexa Fluor® 488 anti-mouse CD28 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1212	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

A20 cells and recombinant mCD28Ig

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 488 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 10⁶ cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: in vitro costimulation of T and NK cells¹, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity, and in vitro induction of thymocyte differentiation. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 122004).

Application References (PubMed link indicates BioLegend citation)

Dennehy KM, et al. 2006. J. Immunol. 176:5725.

RRID

AB_604065 (BioLegend Cat. No. 122017)

Antigen Details

Structure: Ig superfamily, 44 kD
Distribution: Thymocytes, CD4⁺, CD8⁺ peripheral T cells, NK cells
Function: Costimulates T and NK cells
Ligand/Receptor: CD80 (B7-1), CD86 (B7-2)
Cell Type: NK cells, T cells, Thymocytes, Tregs
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.
Gene ID: 12487 View all products for this Gene ID
UniProt: View information about CD28 on UniProt.org

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