PE anti-human CD195 (CCR5) Phospho (Ser349) Antibo
- 产品名称：
- PE anti-human CD195 (CCR5) Phospho (Ser349) Antibo
- 产品类别：
- 抗体
- 产品编号：
- 321606
- 产品应用：
- 321606
[价格]
| 规格 |
价格 |
库存 |
| 100tests |
￥ 3804 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) of CCR5
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The CD195 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The E11/19 monoclonal antibody binds to phosphoserine 349 corresponding to 22 c-terminal amino acid residues of human CCR5 and is useful for flow cytometric analysis, immunoblotting, and ELISA assay of CCR5 expression. Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1, and ELISA detection1 when paired with T21/8 as the capture antibody (Cat. No. 321401/321402).
- Application References
(PubMed link indicates BioLegend citation) -
- Pollok-Kopp B, et al. 2003. J. Biol. Chem. 278:2190. (IF ELISA)
- Fox JM, et al. 2011. 117:1851. PubMed
- Scalley-Kim ML, et al. 2012. PLoS One. 7:e43332. PubMed.
- Product Citations
-
- Dahl M, et al. 2008. J Pharmacol Exp Ther. 327:926. PubMed
- Scalley-Kim M, et al. 2012. PLoS One. 7:e43332. PubMed
- Huffman A,et al. 2017. Biol Blood Marrow Transplant. . 10.1016/j.bbmt.2017.10.028. PubMed
- RRID
- AB_528973 (BioLegend Cat. No. 321606)
- Structure
- G-coupled receptor family 1, membrane protein, 45 kD
- Distribution
-
Subset of T cells, monocytes
- Function
- Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1.
- Ligand/Receptor
- MIP-1a, MIP-1?, RANTES
- Cell Type
- Monocytes, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR, Phospho-Proteins
- Antigen References
-
1. Samson M, et al. 1996. Biochemistry 35:3362.
2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161.
3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147.
4. Deng H, et al. 1996. Nature 381:661.
- Gene ID
- 1234 View all products for this Gene ID
- UniProt
- View information about CD195 Phospho Ser349 on UniProt.org
