Purified anti-human CD35 Antibody
- 产品名称：
- Purified anti-human CD35 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 333402
- 产品应用：
- 333402
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IP, IHC - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
- Application References
(PubMed link indicates BioLegend citation) -
- Hogg N, et al. 1984. Eur. J. Immunol. 14:236
- Zabeleta J, et al. 1998. Clin Diag. Lab. Immunol. 5:690
- Pascual M, et. al. 1994. Eur. J. Immunol. 24:702
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Weinhage T, et al. 2020. J Immunol. 205:56. PubMed
- Syrimi E, et al. 2021. iScience. 24:103215. PubMed
- Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
- RRID
- AB_1089032 (BioLegend Cat. No. 333402)
- Structure
- Type I glycoprotein with four allotypes, 160kD, 190kD, 220kD, 250kD
- Distribution
-
Granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells, subsets of NK and T cells
- Function
- Adhesion, mediate phagocytosis, inhibit T-cell proliferation
- Ligand/Receptor
- C3b, iC3, C4b, iC4
- Cell Type
- B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS
2. Klickstein LB, et al. 1988. J. Exp. Med. 168:1699
- Gene ID
- 1378 View all products for this Gene ID
- UniProt
- View information about CD35 on UniProt.org
