PE anti-human CD35 Antibody
- 产品名称：
- PE anti-human CD35 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 333405
- 产品应用：
- 333405
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1464 |
1 |
- Verified Reactivity
- Human
- Reported Reactivity
- African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
SB -?Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: spatial biology (IBEX)4,5.
- Additional Product Notes
Iterative Bleaching Extended multi-pleXity (IBEX) is a fluorescent imaging technique capable of highly-multiplexed spatial analysis. The method relies on cyclical bleaching of panels of fluorescent antibodies in order to image and analyze many markers over multiple cycles of staining, imaging, and, bleaching. It is a community-developed open-access method developed by the Center for Advanced Tissue Imaging (CAT-I) in the National Institute of Allergy and Infectious Diseases (NIAID, NIH).
- Application References
(PubMed link indicates BioLegend citation) -
- Hogg N, et al. 1984. Eur. J. Immunol. 14:236
- Zabeleta J, et al. 1998. Clin Diag. Lab. Immunol. 5:690
- Pascual M, et. al. 1994. Eur. J. Immunol. 24:702
- Radtke AJ,?et al. 2020.?Proc Natl Acad Sci USA. 117:33455-33465. (SB)?PubMed
- Radtke AJ,?et al. 2022.?Nat Protoc. 17:378-401. (SB)?PubMed
- Product Citations
-
- Stojkov D, et al. 2017. J Cell Biol. 216:4073. PubMed
- Flores E, et al. 2017. Infect Immun . 10.1128/IAI.01043-16. PubMed
- Krampert L, et al. 2021. Front Immunol. 12:712948. PubMed
- Bakke S,et al. 2017. J Immunol. 10.4049/jimmunol.1700302. PubMed
- Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed
- Crane A, et al. 2018. Alzheimers Dement. 14:243. PubMed
- Ozuna H, et al. 2021. Front Immunol. 12:707096. PubMed
- RRID
- AB_2085021 (BioLegend Cat. No. 333405) AB_2292231 (BioLegend Cat. No. 333406)
- Structure
- Type I glycoprotein with four allotypes, 160kD, 190kD, 220kD, 250kD
- Distribution
-
Granulocytes, monocytes, B cells, erythrocytes, follicular dendritic cells, subsets of NK and T cells
- Function
- Adhesion, mediate phagocytosis, inhibit T-cell proliferation
- Ligand/Receptor
- C3b, iC3, C4b, iC4
- Cell Type
- B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
- Biology Area
- Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules
- Antigen References
-
1. Zola Heddy, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD markers. WILEY-LISS
2. Klickstein LB, et al. 1988. J. Exp. Med. 168:1699
- Gene ID
- 1378 View all products for this Gene ID
- UniProt
- View information about CD35 on UniProt.org
