Brilliant Violet 421™ anti-human CD158e1 (KI
- 产品名称：
- Brilliant Violet 421™ anti-human CD158e1 (KI
- 产品类别：
- 抗体
- 产品编号：
- 312713
- 产品应用：
- 312713
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2316 |
1 |
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human NK cell clone VL186-1.6
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421? under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
Brilliant Violet 421? excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 312710).
- Application References
(PubMed link indicates BioLegend citation) -
- Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP)
- Gumperz J, et al. 1996. J. Exp. Med. 183:1817.
- Gardiner CM, et al. 2001. J. Immunol. 166:2992.
- Bakker ABH, et al. 1998. J. Immunol. 160:5239.
- Goodier M, et al. 2000. J. Immunol. 165:139.
- Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC)
- Yawata M, et al. 2002. Immunogenetics 54:543.
- Valiante NM, et al. 1997. Immunity 7:739.
- Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed
- Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed
- Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
- Purdy AK, et al. 2014. J Immunol. 193:4675. PubMed
- Product Citations
-
- Marquardt N, et al. 2015. J Immunol. 195: 3262 - 3272. PubMed
- Li H, et al. 2018. PLoS Genet. 14:e1007163. PubMed
- Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
- Lisovsky I, et al. 2015. J Virol. 89: 9909 - 9919. PubMed
- Guo M, et al. 2021. Front Oncol. 11:632540. PubMed
- Lutter L, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1567. PubMed
- Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
- Lisovsky I, et al. 2015. J Leukoc Biol. 97:761. PubMed
- Isitman G, et al. 2016. PLoS One. 11:e0164517. PubMed
- Boudreau J, et al. 2014. PLoS One. 9:99543. PubMed
- Terszowski G, et al. 2014. J Immunol. 192:5618. PubMed
- Purdy A, et al. 2014. J Immunol. 193:4675. PubMed
- RRID
- AB_10897807 (BioLegend Cat. No. 312713) AB_2561652 (BioLegend Cat. No. 312714)
- Structure
- Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
- Distribution
-
Subset of NK and T cells
- Function
- Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
- Ligand/Receptor
- HLA-B antigens such as the HLA-Bw4 allele
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Colonna M, et al. 1995. Science 268:405.
2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306.
3. Uhrburg M, et al. 1997. Immunity 7:753.
4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817.
5. Wagtmann N, et al. 1995. Immunity 3:801.
- Gene ID
- 3811 View all products for this Gene ID
- UniProt
- View information about CD158e1 on UniProt.org
