Alexa Fluor® 700 anti-human CD158e1 (KIR3DL1,
- 产品名称：
- Alexa Fluor® 700 anti-human CD158e1 (KIR3DL1,
- 产品类别：
- 抗体
- 产品编号：
- 312711
- 产品应用：
- 312711
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human NK cell clone VL186-1.6
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.125 ?g per million cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8. The LEAF? purified antibody (Endotoxin <0.1 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for functional assays (Cat. No. 312710).
- Application References
(PubMed link indicates BioLegend citation) -
- Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP)
- Gumperz J, et al. 1996. J. Exp. Med. 183:1817.
- Gardiner CM, et al. 2001. J. Immunol. 166:2992.
- Bakker ABH, et al. 1998. J. Immunol. 160:5239.
- Goodier M, et al. 2000. J. Immunol. 165:139.
- Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC)
- Yawata M, et al. 2002. Immunogenetics 54:543.
- Valiante NM, et al. 1997. Immunity 7:739.
- Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed
- Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed
- Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
- Purdy AK, et al. 2014. J Immunol. 193:4675. PubMed
- Product Citations
-
- Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
- Sekine T, et al. 2016. Blood. 128: 297 - 312. PubMed
- Bj?rkstr?m N, et al. 2011. J Exp Med. 208:13:00. PubMed
- Poznanski SM, et al. 2018. Cancer Immunol Res. 1.065277778. PubMed
- Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
- Cosgrove C, et al. 2014. PLoS One. 9:105950. PubMed
- Fauriat C, et al. 2010. Blood. 115:1166. PubMed
- Miller J, et al. 2014. Biol Blood Marrow Transplant. 20:1252. PubMed
- Bziat V, et al. 2021. Cell. . PubMed
- Foley B, et al. 2012. Blood. 119:2665. PubMed
- Naluyima P, et al. 2019. J Immunol. 203:2210. PubMed
- K?rner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
- Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
- Portillo AL, et al. 2021. iScience. 24(6):102619. PubMed
- Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
- RRID
- AB_2234188 (BioLegend Cat. No. 312711) AB_2130824 (BioLegend Cat. No. 312712)
- Structure
- Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
- Distribution
-
Subset of NK and T cells
- Function
- Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
- Ligand/Receptor
- HLA-B antigens such as the HLA-Bw4 allele
- Cell Type
- NK cells, T cells
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Colonna M, et al. 1995. Science 268:405.
2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306.
3. Uhrburg M, et al. 1997. Immunity 7:753.
4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817.
5. Wagtmann N, et al. 1995. Immunity 3:801.
- Gene ID
- 3811 View all products for this Gene ID
- UniProt
- View information about CD158e1 on UniProt.org
