Ultra-LEAF™ Purified anti-mouse CD183 (CXCR3
- 产品名称：
- Ultra-LEAF™ Purified anti-mouse CD183 (CXCR3
- 产品类别：
- 抗体
- 产品编号：
- 126526
- 产品应用：
- 126526
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse N-terminus of CXCR3
- Formulation
- 0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/?g of the protein (<0.001 ng/?g of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
Block - Reported in the literature, not verified in house
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 ?g per million cells in 100 ?l volume or 100 ?l of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone CXCR3-173 does not work for Western blotting.7?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.
- Application References
(PubMed link indicates BioLegend citation) -
- Krug A, et al. 2002. J. Immunol. 169:6079.
- McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed
- Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed
- Barbi J, et al. 2009. FASEB J. 23:3990. PubMed
- Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed
- Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed
- Uppaluri R, et al. 2008. Transplantation 86:137.
- Product Citations
-
- Tsuchiya N, et al. 2020. Cell Reports. 29(1):162-175.e9.. PubMed
- Ishikura N, et al. 2022. Oncol Rep. 47:. PubMed
- Li X, et al. 2022. Oncoimmunology. 11:2118210. PubMed
- RRID
- AB_11147756 (BioLegend Cat. No. 126526) AB_11147756 (BioLegend Cat. No. 126554) AB_2571999 (BioLegend Cat. No. 126537) AB_2572000 (BioLegend Cat. No. 126538) AB_2832455 (BioLegend Cat. No. 126547) AB_2832456 (BioLegend Cat. No. 126548)
- Distribution
-
T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors
- Function
- Essential in T cell recruitment to sites of inflammation
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Gene ID
- 12766 View all products for this Gene ID
- UniProt
- View information about CD183 on UniProt.org
