Brilliant Violet 510™ anti-mouse CD183 (CXCR
- 产品名称：
- Brilliant Violet 510™ anti-mouse CD183 (CXCR
- 产品类别：
- 抗体
- 产品编号：
- 126527
- 产品应用：
- 126527
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Armenian Hamster
- Immunogen
- Mouse N-terminus of CXCR3
- Formulation
- ?l size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510? under optimal conditions.
- Concentration
- ?g size: 0.2 mg/mL?L size: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?l size, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510? excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510? is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet?.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Clone CXCR3-173 does not work for Western blotting.7?For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF? purified antibody (Cat. No. 126526) with endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered.
- Application References
(PubMed link indicates BioLegend citation) -
- Krug A, et al. 2002. J. Immunol. 169:6079.
- McAleer JP, et al. 2009. J. Immunol. 182:5322. PubMed
- Sharma R. 2009. J. Immunol. 183:3212. (FC) PubMed
- Barbi J, et al. 2009. FASEB J. 23:3990. PubMed
- Kimpfler S, et al. 2009. J. Immunol. 183:6330. PubMed
- Dürr C, et al. 2010. Cancer Res. 70:10170. (Block) PubMed
- Uppaluri R, et al. 2008. Transplantation 86:137.
- Product Citations
-
- Sagnella SM, et al. 2020. Cancer Cell. 37(3):354-370. PubMed
- Hayatsu N et al. 2017. Immunity. 47(2):268-283 . PubMed
- Kelsey E Sivick et al. 2018. Cell reports. 25(11):3074-3085 . PubMed
- Kyburz A, et al. 2019. J Allergy Clin Immunol. 143:1496. PubMed
- Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
- Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
- RRID
- AB_2562204 (BioLegend Cat. No. 126527) AB_2650922 (BioLegend Cat. No. 126528)
- Distribution
-
T cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors
- Function
- Essential in T cell recruitment to sites of inflammation
- Cell Type
- Dendritic cells, Hematopoietic stem and progenitors, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Gene ID
- 12766 View all products for this Gene ID
- UniProt
- View information about CD183 on UniProt.org
