Ultra-LEAF™ Purified anti-mouse CD314 (NKG2D-上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2376	1

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

Mouse NKG2D

Formulation

0.2 ?m filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is < 0.01 EU/?g of the protein (< 0.001 ng/?g of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF? (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF? solution contains no preservative; handle under aseptic conditions.

Application

FC - Quality tested
Block - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per million cells in 100 ?L volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

To reduce non-specific binding, preincubate cells with purified anti-mouse CD16/CD32, clone 93 (Cat No 101301/2) is recommended.

This product is for in vitro research use only. It is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes contact The Regents of the University of California.

Application References (PubMed link indicates BioLegend citation)

Ogasawara K, et al. 2003. Immunity 18:41. (Block)
Ogasawara K, et al. 2004. Immunity 20:757. (Block)
Kong LY, et al. 2010. Clin. Cancer Res. 16:2550. PubMed

RRID

AB_2832469 (BioLegend Cat. No. 130217) AB_2832470 (BioLegend Cat. No. 130218)

Antigen Details

Structure: NKG2D is a lectin-like type II transmembrane protein. It serves as a stimulatory receptor to activate NK cells via the non-covalently associated DAP10 or DAP 12 daptor protein.
Distribution: NK, NKT cells
Ligand/Receptor: RAE, H60,
Cell Type: NK cells, NKT cells
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Gene ID: 27007 View all products for this Gene ID
UniProt: View information about CD314 on UniProt.org

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