Go-ChIP-Grade™ Purified anti-RNA Polymerase -上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 996	1

Product Details

Verified Reactivity

Human, Mouse, Rat, Drosophilia, Yeast (S. cerevisiae), Zebrafish

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

The immunogen used was a peptide containing 10 repeats of the synthetic peptide YSPTSPS using chemically synthesized phospho-ser5.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

ChIP -?Quality tested
WB -?Verified
ICC?-?Reported in the literature, not verified in house?

Recommended Usage

Each lot of this antibody is quality control tested by ChIP Assay. The suggested dilution for ChIP application is 1:300-1:500 by volume.?For Western blotting the suggested working dilution is?1:500 by volume. For immunofluorescent staining, the suggested dilution is 1:500 by volume.

It is recommended that the reagent to be titrated for optimal performance before each experiment.

Application Notes

This antibody is effective in immunoblotting, immunoprecipitation and ELISA. It can also be used in chromatin immunoprecipitation assays.

The antibody CTD4H8 recognizes the C-terminal repeat of the largest subunit of RNA polymerase II from HeLa and S. cerevisiae cells. This antibody recognizes both the phosphorylated and unphosphorylated forms of the RNA polymerase II.

Based on sequence identify, this clone is liable to react with a broad range of species.

Clone CTD4H8 is also known as 4H8 or CTD 4H8.

Predicted MW is ~ 217 kD, Observed MW is on WB gel is ~240 kD

Application References (PubMed link indicates BioLegend citation)