Alexa Fluor® 647 anti-RNA Polymerase II Antibo-上海雅吉生物科技有限公司

规格	价格	库存
25µg	￥ 1908	1

Product Details

Verified Reactivity

Human, Mouse, Rat, Drosophilia, Yeast (S. cerevisiae), Zebrafish

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

The immunogen used was a peptide containing 10 repeats of the synthetic peptide YSPTSPS using chemically synthesized phospho-ser5.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 647 under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

ICC -?Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 1 - 2 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

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Excitation Laser

Red Laser (633 nm)

Application Notes

This antibody is effective in immunoblotting, immunoprecipitation and ELISA. It can also be used in chromatin immunoprecipitation assays.

The antibody CTD4H8 recognizes the C-terminal repeat of the largest subunit of RNA polymerase II from HeLa and?S. cerevisiae?cells. This antibody recognizes both the phosphorylated and unphosphorylated forms of the RNA polymerase II.

Based on sequence identify, this clone is liable to react with a broad range of species.

Clone CTD4H8 is also known as 4H8 or CTD 4H8.

Predicted MW is ~ 217 kD, Observed MW is on WB gel is ~240 kD

Application References (PubMed link indicates BioLegend citation)