Purified anti-O-GlcNAc Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 4164	1

Product Details

Verified Reactivity

All Species

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

The antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C.

Application

WB - Quality tested
ICC, IHC-P - Verified
IP?- Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL?is recommended.?For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 10 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.

Additional reported applications (for the relevant formats) include: ELISA², immunocytochemistry⁷, immunoprecipitation^2,5,6, and Western blotting^3,4,7.

Application References (PubMed link indicates BioLegend citation)

Comer FI, et al. 2001. Anal. Biochem. 293:169.
Hart GW, et al. 1995. Adv. Exp. Med. Biol. 376:115. (ELISA, WB, IP)
Anthonisen EH, et al. 2010. J. Biol. Chem. 285:1607. (WB)
Dias WB, et al. 2009. J. Biol. Chem. 32:21327. (WB)
Wang Z, et al. 2007. Mol. Cell Proteomics 8:1365. (IP)
Kim SJ, et al. 2003. J. Biol. Chem. 43:41890. (IP)
Kneass ZT, Marchase RB. 2004. J. Biol. Chem. 44:45759. (WB, ICC, IF)

Product Citations

Durst MA, et al. 2019. PLoS One. 14:e0210305. PubMed
Kuo WL, et al. 2021. Diagnostics (Basel). 11:. PubMed
Liu Y, et al. 2020. Nat Commun. 4.554166667. PubMed
Basehore SE, et al. 2021. Circ Res. 129:1054. PubMed
Jean-Louis T, et al. 2018. Neurobiol Aging. 62:130. PubMed

RRID

AB_2629520 (BioLegend Cat. No. 838004)

Antigen Details

Structure: 1046 amino acids with a molecular weight of 117 kD.
Distribution: Cytosol, mitochondrion, nucleus, plasma membrane, and cytoskeleton.
Function: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc), Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1, Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.
Biology Area: Cell Biology, Transcription Factors
Gene ID: 8473 View all products for this Gene ID
UniProt: View information about O-GlcNAc on UniProt.org

上海细胞库

原代细胞

细胞培养

细胞服务

酶联试剂盒

生化试剂盒

PCR试剂盒

抗体蛋白

试剂耗材

首页 > 抗体蛋白 > 抗体

产品详情

Product Details

Antigen Details

联系我们