Alexa Fluor® 594 anti-O-GlcNAc Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 4860	1

Product Details

Verified Reactivity

All Species

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

The antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 594 under optimal conditions.

Concentration

0.5 mg/mL

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

IHC-P?- Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, the suggested use of this reagent is 5.0 - 10 ?g/mL. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor? 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.

Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.

View full statement regarding label licenses

Excitation Laser

Green Laser (532 nm)/Yellow-Green Laser (561 nm)

Application Notes

This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.

Additional reported applications (for the relevant formats) include: ELISA², immunocytochemistry⁷, immunoprecipitation^2,5,6, and Western blotting^3,4,7.

Application References (PubMed link indicates BioLegend citation)

Comer FI, et al. 2001. Anal. Biochem. 293:169.
Hart GW, et al. 1995. Adv. Exp. Med. Biol. 376:115. (ELISA, WB, IP)
Anthonisen EH, et al. 2010. J. Biol. Chem. 285:1607. (WB)
Dias WB, et al. 2009. J. Biol. Chem. 32:21327. (WB)
Wang Z, et al. 2007. Mol. Cell Proteomics 8:1365. (IP)
Kim SJ, et al. 2003. J. Biol. Chem. 43:41890. (IP)
Kneass ZT, Marchase RB. 2004. J. Biol. Chem. 44:45759. (WB, ICC, IF)

RRID

AB_2632636 (BioLegend Cat. No. 838006)

Antigen Details

Structure: 1046 amino acids with a molecular weight of 117 kD.
Distribution: Cytosol, mitochondrion, nucleus, plasma membrane, and cytoskeleton.
Function: Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc), Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1, Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.
Biology Area: Cell Biology, Transcription Factors
Gene ID: 8473 View all products for this Gene ID
UniProt: View information about O-GlcNAc on UniProt.org

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