PE/Fire™ 810 anti-human CD28 Antibody
- 产品名称：
- PE/Fire™ 810 anti-human CD28 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302971
- 产品应用：
- 302971
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 2328 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE/Fire? 810 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 ?L per million cells in 100 ?L staining volume or 5 ?L per 100 ?L of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* PE/Fire? 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire? 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for highly sensitive assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Nunes J, et al. 1993. Biochem. J. 293:835.
- Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
- Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
- Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
- Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
- Rivollier A, et al. 2004. Blood 104:4029. (Costim)
- Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
- Sheng W, et al. 2007.Elsevier 580:6819. PubMed
- Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
- Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
- Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
- Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC)
- Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed
- Nomura T, et al. 2012. J. Virol. 86:6481. PubMed
- RRID
- AB_2894559 (BioLegend Cat. No. 302971)
- Structure
- Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
- Distribution
-
Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD80, CD86
- Cell Type
- B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321.
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
- Gene ID
- 940 View all products for this Gene ID
- UniProt
- View information about CD28 on UniProt.org
