PE anti-human CD28 Antibody
- 产品名称：
- PE anti-human CD28 Antibody
- 产品类别：
- 抗体
- 产品编号：
- 302907
- 产品应用：
- 302907
[价格]
| 规格 |
价格 |
库存 |
| 25tests |
￥ 1188 |
1 |
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- Baboon, Capuchin Monkey, Chimpanzee, Pigtailed Macaque, Sooty Mangabey, Squirrel Monkey
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- ?g size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA). - Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- ?g sizes: 0.2 mg/mLtest sizes: lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the ?g size, the suggested use of this reagent is ≤0.5 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 ?l per million cells in 100 ?l staining volume or 5 ?l per 100 ?l of whole blood.
- Excitation Laser
- Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
The Ultra-LEAF? Purified antibody (Endotoxin < 0.01 EU/?g, Azide-Free, 0.2 ?m filtered) is recommended for highly sensitive assays.
- Application References
(PubMed link indicates BioLegend citation) -
- Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
- Nunes J, et al. 1993. Biochem. J. 293:835.
- Calea-Lauri J, et al. 1999. J. Immunol. 163:62.
- Tazi A, et al. 1999. J. Immunol. 163:3511. (IHC)
- Marti F, et al. 2001. J. Immunol. 166:197. (Costim)
- Jeong SH, et al. 2004. J. Virol. 78:6995. (Costim)
- Rivollier A, et al. 2004. Blood 104:4029. (Costim)
- Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Costim)
- Sheng W, et al. 2007.Elsevier 580:6819. PubMed
- Mitsuhashi M. 2007. Clin Chem.53:148. PubMed
- Ye Z, et al. 2008. Infect. Immun. 76:2541. PubMed
- Magatti M, et al. 2008. Stem Cells 26:182. (FA) PubMed
- Yoshino N, et al. 2008. Exp. Anim. (Tokyo) 49:97. (FC)
- Berg M, et al. 2008. J Leukoc Biol. 83:853. (IP) PubMed
- Rout N, et al. 2010. PLoS One 5:e9787. (FC)
- Leonard JA, et al. 2011. J. Virol. 85:6867. PubMed
- Nomura T, et al. 2012. J. Virol. 86:6481. PubMed
- Product Citations
-
- Andonian BJ, et al. 2022. Sci Rep. 12:7450. PubMed
- Weiskopf D, et al. 2015. Proc Natl Acad Sci U S A. 112: E4256-E4263. PubMed
- Wu HL, et al. 2018. J Immunol. 200:49. PubMed
- Beatson RE, et al. 2021. Cell Rep Med. 2:100473. PubMed
- Zhou WJ, et al. 2021. Theranostics. 11:5330. PubMed
- Garimilla S, et al. 2019. JCI Insight. 4:9. PubMed
- Hunter S, et al. 2018. J Hepatol. 69:654. PubMed
- Zebley CC, et al. 2021. Cell Rep. 37:110079. PubMed
- Hirama T, et al. 2021. JCI Insight. 6:. PubMed
- Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
- Yang ZZ, et al. 2019. Cell Rep. 26:2178. PubMed
- Kalim H, et al. 2020. Int J Rheum Dis. 23:620. PubMed
- Wang X, et al. 2022. J Exp Clin Cancer Res. 41:210. PubMed
- Fu J, et al. 2021. J Clin Invest. 131:. PubMed
- Alhaj Hussen K, et al. 2020. Front Immunol. 11:579776. PubMed
- Estrada-Capetillo L, et al. 2021. Eur J Immunol. 51:824. PubMed
- Davis-Marcisak EF, et al. 2021. Genome Med. 13:129. PubMed
- RRID
- AB_314309 (BioLegend Cat. No. 302907) AB_314310 (BioLegend Cat. No. 302908) AB_2564147 (BioLegend Cat. No. 302940)
- Structure
- Ig superfamily, type I transmembrane glycoprotein, homodimer, 44 kD
- Distribution
-
Mature T cells, thymocytes, NK cell subsets, plasma cells, EBV-positive B cells
- Function
- T cell costimulation
- Ligand/Receptor
- CD80, CD86
- Cell Type
- B cells, NK cells, Plasma cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
-
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321.
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.
- Gene ID
- 940 View all products for this Gene ID
- UniProt
- View information about CD28 on UniProt.org
