Biotin anti-human CD47 Antibody -上海雅吉生物科技有限公司

规格	价格	库存
100µg	￥ 2532	1

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Cynomolgus, Rhesus

Antibody Type

Monoclonal

Host Species

Mouse

Immunogen

CCRF-CEM T-cell line

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 ?g per million cells in 100 ?l volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The CC2C6 monoclonal antibody can block the binding of HCD47 antibody to CD47.

Additional reported applications (for the relevant formats) include: blocking²

Application References (PubMed link indicates BioLegend citation)

Seiffert M, et al. 1999. Blood 94:3633.
Leclair P, et al. 2018. Cell Death Dis. 5:544 (Block)

Product Citations

Mair B et al. 2019. Nat Biomed Eng. 3(10):796-805 . PubMed
Zaborowski MP et al. 2019. Cell reports. 27(1):255-268 . PubMed
Lamanna J, et al. 2020. Nat Commun. 4.369444444. PubMed
Kaur S, et al. 2014. J Immunol. 193:3914. PubMed

RRID

AB_756134 (BioLegend Cat. No. 323104)

Antigen Details

Structure: Member of the immunoglobulin superfamily, 42-52 kD protein with a five-pass transmembrane attachment. Two splice variants have been described in the cytoplasmic tail.
Distribution: Wide distribution in hematopoietic cells including thymocytes, T cells, B cells, platelets, and erythrocytes as well as epithelial cells, endothelial cells, fibroblasts, and neural tissues.
Function: Adhesion molecule and thrombospondin receptor. In the absence of CD47, mice show defects in host defense and beta 3 integrin-dependent ligand binding, migration, and cellular activation. CD47 is a part of the Rh complex on erythrocytes.
Ligand/Receptor: Non-covalently associated with ?3 integrins CD51/CD61, CD41/CD61. Thrombospondin is a ligand for CD47.
Cell Type: B cells, Endothelial cells, Epithelial cells, Erythrocytes, Fibroblasts, Platelets, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Anstee DJ, et al. 1995. In Leucocyte Typing V (Schlossman ed.) Oxford University Press Oxford pp233-234.
2. Brown E, et al. 1990. J. Cell Biol. 111:2785.
3. Gao AG, et al. 1996. J. Biol. Chem. 271:21.
4. Lindberg FP, et al. 1994. J. Biol. Chem. 269:1567.
Gene ID: 961 View all products for this Gene ID
UniProt: View information about CD47 on UniProt.org

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