Pacific Blue™ anti-human Granzyme A Antibody
- 产品名称：
- Pacific Blue™ anti-human Granzyme A Antibody
- 产品类别：
- 抗体
- 产品编号：
- 507208
- 产品应用：
- 507208
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified human Granzyme A
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with Pacific Blue? under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue? has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue? conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.
- Application References
(PubMed link indicates BioLegend citation) -
- Trimble L, et al. 1998. Blood 91:585.
- Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
- Raqib R, et al. 2002. Infect. Immun. 70:3199.
- Chen H, et al. 2005. J. Immunol. 175:591.
- Product Citations
-
- Dimova T, et al. 2015. Proc Natl Acad Sci U S A. 112:556. PubMed
- Kiniry BE, et al. 2018. J Immunol. 200:1876. PubMed
- Yamamoto T, et al. 2012. J Virol. 86:5877. PubMed
- Sanchez-Ruiz Y, et al. 2011. PLoS One. 6:e27057. PubMed
- Marquardt N, et al. 2015. J Immunol. 194:2467. PubMed
- Cellerai C, et al. 2010. J Virol. 84:3868. PubMed
- RRID
- AB_492996 (BioLegend Cat. No. 507208) AB_439755 (BioLegend Cat. No. 507207)
- Structure
- Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
- Bioactivity
- Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
- Cell Sources
- Cytotoxic T cells, NK cells
- Cell Targets
- Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
- Receptors
- Assisted by perforin
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Proteases, Enzymes and Regulators
- Antigen References
-
1. Brune J, et al. 1986. Nature 322:268.
2. Fan Z, et al. 2003. Nature Immunol. 4:145.
3. Fan Z, et al. 2003. Cell 112:659.
4. Masson D, et al. 1987. Cell 49:679.
- Gene ID
- 3001 View all products for this Gene ID
- UniProt
- View information about Granzyme A on UniProt.org
