Alexa Fluor® 700 anti-human Granzyme A Antibod
- 产品名称：
- Alexa Fluor® 700 anti-human Granzyme A Antibod
- 产品类别：
- 抗体
- 产品编号：
- 507210
- 产品应用：
- 507210
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Purified human Granzyme A
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor? 700 under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.5 ?g per 106 cells in 100 ?l volume. It is highly recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor? 700 has a maximum emission of 719 nm when it is excited at 633nm / 635nm. Prior to using Alexa Fluor? 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor? and Pacific Blue? are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
- Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemical staining3 of formalin-fixed paraffin-embedded tissue sections, and immunoprecipitation2.
- Application References
(PubMed link indicates BioLegend citation) -
- Trimble L, et al. 1998. Blood 91:585.
- Beresford P, et al. 1997. P. Natl. Acad. Sci. USA 94:9285.
- Raqib R, et al. 2002. Infect. Immun. 70:3199.
- Chen H, et al. 2005. J. Immunol. 175:591.
- Product Citations
-
- Leeansyah E, et al. 2015. PLoS Pathog. 11: 1005072. PubMed
- Hersperger A, et al. 2011. Blood. 117:3799. PubMed
- Mold JE, et al. 2021. Cell Reports. 35(8):109174. PubMed
- Cheng J, et al. 2022. iScience. 25:103588. PubMed
- Katsuyama E, et al. 2020. Cell Reports. 30(1):112-123.e4.. PubMed
- RRID
- AB_961343 (BioLegend Cat. No. 507210)
- Structure
- Serine protease; disulfide-linked homodimer; 28 kD (Mammalian)
- Bioactivity
- Induction of caspase-independent cell death by apoptosis; release of NM23-H1 for single-strand DNA nicking
- Cell Sources
- Cytotoxic T cells, NK cells
- Cell Targets
- Intracellular targets lamins A, B cells, C, nucleosome assembly protein (NAP) SET cells, HMG2, Ape1/Ref-1, histones; cleaves SET:NM23-H1 complex to release NM23-H1
- Receptors
- Assisted by perforin
- Cell Type
- Tregs
- Biology Area
- Cell Biology, Immunology, Innate Immunity, Neuroscience
- Molecular Family
- Proteases, Enzymes and Regulators
- Antigen References
-
1. Brune J, et al. 1986. Nature 322:268.
2. Fan Z, et al. 2003. Nature Immunol. 4:145.
3. Fan Z, et al. 2003. Cell 112:659.
4. Masson D, et al. 1987. Cell 49:679.
- Gene ID
- 3001 View all products for this Gene ID
- UniProt
- View information about Granzyme A on UniProt.org
